AN EFFICIENT RECOVERY OF TRANSGENIC PLANTS FROM A TROPICAL INDIAN MAIZE INBRED LINE

J. Beslin Joshi; G. Nallathambi; K.K. Kumar; E. Kokiladevi; L. Arul; P. Balasubramanian; D. Sudhakar

doi:10.15414/jmbfs.2016.5.4.335-340

AN EFFICIENT RECOVERY OF TRANSGENIC PLANTS FROM A TROPICAL INDIAN MAIZE INBRED LINE

Authors

J. Beslin Joshi
G. Nallathambi
K.K. Kumar
E. Kokiladevi
L. Arul
P. Balasubramanian
D. Sudhakar

DOI:

https://doi.org/10.15414/jmbfs.2016.5.4.335-340

Keywords:

Maize, particle bombardment, gusA, bar, transgenic plants

Abstract

Tropical Indian maize inbreds are known for their recalcitrance in tissue culture which is a prerequisite for development of transgenic lines from such cultivars. In the present study, particle bombardment-mediated transformation of a tropical Indian maize inbred line, UMI29 was attempted. Parameters that infulence transforamation efficiency such as size of embryo (1.5 mm and 2.0 mm), micro-carrier flying distance (6 cm and 9 cm) and post-bombardment resting period (2, 7 and 10 days) on transient and stable expression of transgene were investigated. The greatest transformation efficiency of 2.67% was obtained using immature embryos of 1.5 mm at 6 cm of microcarrier flying distance with a resting period of 2 days. Stable inheritance of transgenes, viz., gusA and bar was confirmed in T1 lines.

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Published

2016-02-01

How to Cite

Beslin Joshi, J., Nallathambi, G., Kumar, K., Kokiladevi, E., Arul, L., Balasubramanian, P., & Sudhakar, D. (2016). AN EFFICIENT RECOVERY OF TRANSGENIC PLANTS FROM A TROPICAL INDIAN MAIZE INBRED LINE. Journal of Microbiology, Biotechnology and Food Sciences, 5(4), 335–340. https://doi.org/10.15414/jmbfs.2016.5.4.335-340

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Issue

Vol. 5 No. 4 (2016): February - March

Section

Biotechnology

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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