ISOLATION AND CHARACTERIZATION OF TANNASE PRODUCING BACTERIA FROM THE GUT OF GRYLLOTALPA KRISHNANI

Abstract

Gryllotalpa krishnani mainly eats the degradable food waste, so the insect gut which may contains an interesting diversity of microorganism not usually found elsewhere. The tannase enzymes have been reported for its wider applications in food, feed, beverage, pharmaceutical, and chemical industries. In the present study,microorganism were isolated from the gut of Gryllotalpa krishnani and characterized for tannase activity. A total number of 40 bacterial strains were isolated and checked for tannase activity. Among them, 4 strains produced high level of tannase activity in plate assay. Further, to confirm the identify of bacterial isolates strains were subjected biochemical and 16S rDNA sequencing analysis. The results confirmed the identity if the isolates belonging to Enterobacter cloacae (15), Bacillus subtilis (25), Enteroinbacteriaceae bacterium (39) and Bacillus cereus (40). The four bacterial strain where subjected for the substrate utilization. Interestingly, all the isoaltes showed clearing zone upto 2% tannic acid. Tannic acid degrading microbiota detected in the present study may endow the insect with some ecological advantages by enabling them to overcome the anti-nutritional effects of plant tannins.