ASSESSMENT OF HEMATOLOGICAL AND BIOCHEMICAL EFFECTS OF ACUTE AND CHRONIC ADMINISTRATION OF THE STENOTROPHOMONAS MALTOPHILIA LYSATES IN MICE

Abstract

The contamination of rice with microorganisms during the storage is very common. In the present study we observe the effect of consumption of such contaminated rice and the lysate obtained from the microorganism on the hematological and biochemical parameters. Among all the microorganisms the bacteria exhibiting gross alteration on the locomotor effect was identified by the 16s rRNA sequencing of the bacteria which is found to be Stenotrophomonas maltophilia. The lysate obtained from the bacterial strain gave an LD50 value more than 2000 mg/kg p.o. Though acute exposure to the lysate reduced the locomotor activity but no such effect was observed for the chronic exposure. The study exhibited that at 200 mg/kg p.o. and 300 mg/kg p.o. for a period of 15 days administration the hematological and biochemical profile was unchanged. Though at chronic exposure of 200 mg/kg p.o. there was no change in the hematological and biochemical parameters but a long term exposure of the lysate at 300 mg/kg p.o. reduced hemoglobin and red blood corpuscles count. Moreover, at the higher dose exposure there was an increase in the liver enzymes Alanine aminotransferase (ALT), aspartate aminotranferase (AST) and alkaline phosphatase (ALP). Thus, it indicated that at higher dose long term exposure the lysate of Stenotrophomonas maltophilia may be responsible for toxic changes in the body.