EXPLORATION OF ANTIBACTERIAL AND ANTIPROLIFERATIVE SECONDARY METABOLITES FROM MARINE BACILLUS

Authors

  • Chandini S. Syed
  • Mantri Sairam
  • Amrutha V. Audipudi

DOI:

https://doi.org/10.15414/jmbfs.2019/20.9.3.628-633

Keywords:

Antibacterial, antiproliferative, A-549 cell line, Bacillus flexus AVSC4, GC-MS analysis, HT-29 cell line

Abstract

Antiproliferative secondary metabolites producing bacterial strain AVSC4 isolated from marine sediments was identified as Bacillus flexus based on 16S rRNA gene sequence analysis. Under the strategy of liquid- liquid extraction, the crude extract was obtained showed significant antibacterial activity against different clinical pathogens. 0.5% methionine and 0.4% NaCl act as inducers for maximizing the growth and antibacterial activity of strain at pH 7 and 40°C. Heptadecanoic acid and methyl hexadecanoic acid were identified as major and dominant secondary metabolites by GC-MS analysis and also showed significant antiproliferative activity against HT-29 (Human colorectal adenocarcinoma) and A-549 (Lung Cancer) with IC50 value 93.4 µg/ml and 50.04 µg/ml.

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Published

2019-12-01

How to Cite

S. Syed, C., Sairam, M., & V. Audipudi, A. (2019). EXPLORATION OF ANTIBACTERIAL AND ANTIPROLIFERATIVE SECONDARY METABOLITES FROM MARINE BACILLUS. Journal of Microbiology, Biotechnology and Food Sciences, 9(3), 628–633. https://doi.org/10.15414/jmbfs.2019/20.9.3.628-633