EFFECT OF GERMINATION ON FREE RADICAL SCAVENGING ACTIVITIES AND ANGIOTENSIN I-CONVERTING ENZYME INHIBITORY OF MELINJO (Gnetum gnemon L) SEED PROTEINS

Abstract

The effect of germination on free radical scavenging activities and Angiotensin I-Converting Enzyme (ACE-I) inhibitory of melinjo (Gnetum gnemon) seed proteins was studied to determine its potential use as a nutraceutical ingredient. Investigations into the protein profiles, amino acid composition, free radical scavenging activities and ACE-I-inhibitory of proteins extracted from melinjo seed during germination (0 to 21 days) were carried out. The concentration of amino acids detected in the melinjo seed samples ranged from 0.30 to 12.75 g/100 g protein and the degree of hydrolysis during germination at 21st days was significantly highest than that of either (0-14 days). Furthermore, with all samples a large small-sized peptides degree (<10 kDa) was observed with an increasing degree of hydrolysis value. The free radical scavenging activities were measured using different standard methods, and ACE-I inhibitory activity was determined using in vitro ACE-I inhibitory assay. The time of germination showed a significant effect (p<0.05) on free radical scavenging (ABTS2+, DPPH, O2•, and OH•) activities and ACE-I inhibitory. The above results indicated that germination time has positively affected ACE-I inhibitory activity and free radical scavenging activities.