A COMPUTATIONAL GENOME ANALYSIS OF STRAIN BACILLUS SUBTILIS MIZ-8 ISOLATED FROM BEKANG REVEALS A DISTINCT CHROMOSOME AND PLASMID CONFERRING SELECTIVE ADVANTAGE

Authors

  • Harini Keerthana Suresh Kumar Department of Molecular Medicine,Universitätsklinikum Jena - Faculty of Medicine, Friedrich Schiller Universität Jena, Germany https://orcid.org/0000-0003-2667-0144
  • Trishala Gopikrishna Department of Biotechnology, Sri Ramachandra Faculty of Biomedical Sciences & Technology, Sri Ramachandra Institute of Higher Education and Research (SRIHER), Chennai, India.
  • Tapasya K Department of Biotechnology, Sri Ramachandra Faculty of Biomedical Sciences & Technology, Sri Ramachandra Institute of Higher Education and Research, Chennai, India. https://orcid.org/0000-0002-1788-7619
  • Kumar Perumal Assistant Professor, Department of Biotechnology, Sri Ramachandra Faculty of Biomedical Sciences & Technology, Sri Ramachandra Institute of Higher Education and Research (SRIHER), Chennai, India.
  • Elavarashi E Sri Ramachandra Institute of Higher Education and Research https://orcid.org/0000-0002-7813-6755

DOI:

https://doi.org/10.55251/jmbfs.9141

Keywords:

Bacillus subtilis, Bekang, fermented food, Whole genome sequencing

Abstract

Bacillus subtilis Miz-8 is isolated from Bekang, a traditional fermented soybean food of Mizoram, India. Here, we report computationally analyzed strain Miz-8 mapped to the annotated genome of the reference strain Bacillus subtilis subsp. natto BEST195. Miz-8 has a circular chromosome and a small plasmid with genome size of 4105264 bp and 5838 bp, respectively. Genome contains the genes responsible for synthesis of biosynthetic metabolites like surfactin, fengycin, bacillibactin, bacilysin, subtilosin A and alpha amylase production. The strain Miz-8 harbored virulence genes identical to the strain BEST195 render themselves harmless for human consumption. However, Strain Miz-8 has one intact prophage region but no integrase protein, making it incapable of lateral transfer of antimicrobials. Antibiotic resistance genes were predicted among which tmrB gene was on perfect hit. Plasmid of strain Miz-8 contains no prophage sequences and antibiotic resistance genes. Furthermore, there were several single nucleotide polymorphisms and 344 insertion-deletion polymorphisms. Bacillus subtilis Miz-8's genomic information unmasks its functional significance on human health.

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Published

2023-06-26

How to Cite

Suresh Kumar, H. K., Gopikrishna, T., K, T., Perumal, K., & E, E. (2023). A COMPUTATIONAL GENOME ANALYSIS OF STRAIN BACILLUS SUBTILIS MIZ-8 ISOLATED FROM BEKANG REVEALS A DISTINCT CHROMOSOME AND PLASMID CONFERRING SELECTIVE ADVANTAGE. Journal of Microbiology, Biotechnology and Food Sciences, 13(2), e9141. https://doi.org/10.55251/jmbfs.9141