ANTIBIOTIC RESISTANCE OF ESBL-PRODUCING E. COLI AND OTHER GRAM-NEGATIVE BACTERIA ISOLATED FROM STREET-VENDED FOODS IN BANGLADESH

Abstract

The prevalence and impact of antibiotic-resistant pathogens transmitted through food, particularly street-vended foods, is becoming a major public health concern. Although a significant proportion of the urban population in developing countries consumes street-vended foods, the role of these foods in spreading antibiotic resistance has been rarely investigated. In this study, 50 bacterial isolates were obtained from 25 samples representing five categories of street-vended foods: Phuchka, Chatpati, Sausage, Bun, and Salad.  The IMVIC test revealed a notably high occurrence of Escherichia coli (n=32) within the collected samples. Three representative isolates were selected for molecular identification using DNA sequencing of 16S rDNA. They were identified as Klebsiella oxytoca, Burkholderia fungorum, and Serratia nematodiphila. The antibiotic susceptibility of the identified isolates (n=35) was investigated using twelve antibiotics following the Kirby-Bauer disk diffusion method. Around 65.63% of the E. coli isolates (n=21) exhibited multidrug resistance. Double Disc Synergy Test (DDST) and Phenotypic Confirmatory Disk Diffusion Test (PCDDT) confirmed ESBL production of Eight multidrug-resistant E. coli isolates (38.09%). The multiple antibiotic resistance (MAR) index showed that 22 E. coli isolates had MAR above 0.2, with resistance mostly against oxacillin, ampicillin, and cefuroxime. The Klebsiella oxytoca isolate showed multidrug resistance viz., ampicillin, oxacillin, cefuroxime, and kanamycin. The Burkholderia fungorum isolate showed no distinct inhibition zone against ampicillin and chloramphenicol. Additionally, the Serratia nematodiphila isolate showed no distinct inhibition zone against three antibiotics, including ampicillin, oxacillin, and cefuroxime. These findings might contribute to the knowledge of emerging antibiotic-resistant foodborne pathogens and raise concerns about the safety of street-vended foods in Bangladesh.