SEQUENCE COMPARISON OF MUTE PROMOTERS BETWEEN TWO WHEAT CULTIVARS REVEALED BOTH CONSERVATIVE AND DIVERGED REGIONS

Authors

  • Olha Lakhneko Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine https://orcid.org/0000-0002-6460-1724
  • Anton Stepanenko Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Akademika Zabolotnoho st. 148, 03143 Kyiv, Ukraine
  • Yuliia Rymar Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Akademika Zabolotnoho st. 148, 03143 Kyiv, Ukraine https://orcid.org/0000-0003-3051-3774
  • Mykola Borysyuk Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University, Changjiang West Road 111, Huai’an 223300, China https://orcid.org/0000-0001-5250-9771
  • Bogdan Morgun Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Akademika Zabolotnoho st. 148, 03143 Kyiv, Ukraine

DOI:

https://doi.org/10.55251/jmbfs.9662

Keywords:

in silico analysis, wheat, stomata biogenesis, transcription factor, MUTE, promoter, cis-element

Abstract

Modulation of stomatal biogenesis is one of the means to consider for improving crops' water use efficiency without losing productivity. Transcription factor MUTE is among the critical regulators of stomata biogenesis, particularly of the guard mother cell division. In the current study, we investigated promoter regions of three homoeologous MUTE genes–MUTE-A1, MUTE-B1, and MUTE-D1 from two bread wheat cultivars of different geographic origins. Based on the available sequence of the Chinese Spring genome, MUTE promoters were isolated and sequenced from the Ukrainian cultivar Natalka. Promoter regions of more than 1600 bp upstream of the predicted start codons were cloned as PCR-amplified fragments and analyzed by sequencing. The sequence alignments revealed significant subgenome and cross-cultivar heterogeneity of the analyzed promoters. The analysis showed numerous single nucleotide polymorphisms and large deletions in every Natalka sequence compared with Chinese Spring: 276-bp deletion in MUTE-A1, 205-bp in MUTE-B1, 1291-bp in MUTE-D1. These changes affected a range of cis-regulatory elements (light- and stress-responsive elements, as well as tissue-specific elements) within the investigated DNA sequences. The study sheds new light on the regulatory variation of one of the critical factors in stomata biogenesis, widening our understanding of wheat genome plasticity with potential implications for improving the crop's performance under restricted water conditions.

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Author Biographies

Olha Lakhneko, Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine

Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Akademika Zabolotnoho st. 148, 03143 Kyiv, Ukraine

Department of Molecular Genetics, Junior research fellow

Institute of Plant Genetics and Biotechnology, Plant Science Biodiversity Center, Slovak Academy of Sciences, Akademicka 2, P.O. Box 39A, 950 07 Nitra, Slovakia

Department of Molecular Biology and Biotechnology, Post-doctoctoral scholar

 

Anton Stepanenko, Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Akademika Zabolotnoho st. 148, 03143 Kyiv, Ukraine

Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Akademika Zabolotnoho st. 148, 03143 Kyiv, Ukraine

Department of Molecular Genetics, Research fellow

Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University, Changjiang West Road 111, Huai’an 223300, China

Post-doctoral scholar

Yuliia Rymar, Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Akademika Zabolotnoho st. 148, 03143 Kyiv, Ukraine

Department of Molecular Genetics, doctoral student

Mykola Borysyuk, Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University, Changjiang West Road 111, Huai’an 223300, China

Professor (Full)

Bogdan Morgun, Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Akademika Zabolotnoho st. 148, 03143 Kyiv, Ukraine

Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Akademika Zabolotnoho st. 148, 03143 Kyiv, Ukraine

Department of Molecular Biology, Head of Department

 

Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, Vasylkivska st. 31/17, 03022 Kyiv, Ukraine

Senior research fellow

National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, Prosp. Peremohy, 37, 03056 Kyiv, Ukraine

Department of Biotechnology and Biotechnics, Assistant Professor

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Published

2024-02-29

How to Cite

Lakhneko, O., Stepanenko, A., Rymar, Y., Borysyuk, M., & Morgun, B. (2024). SEQUENCE COMPARISON OF MUTE PROMOTERS BETWEEN TWO WHEAT CULTIVARS REVEALED BOTH CONSERVATIVE AND DIVERGED REGIONS. Journal of Microbiology, Biotechnology and Food Sciences, 13(6). https://doi.org/10.55251/jmbfs.9662

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Section

Miscellaneous