A LIPOSOMAL CONTROLLED DELIVERY SYSTEM FOR PLANTAGO MAJOR L.  EXTRACTS AND THEIR BIOLOGICAL ACTIVITY FOR WOUND HEALING

Ozlem ERDAL ALTINTAS; Pinar AYTAR CELIK

doi:10.55251/jmbfs.9929

Authors

Ozlem ERDAL ALTINTAS Medical Laboratory Techniques Program, Suhut Vocational School Of Health Services, Afyonkarahisar Health Sciences University, 03200, Afyonkarahisar, Turkey
Pinar AYTAR CELIK Eskisehir Osmangazi University https://orcid.org/0000-0002-9447-1668

DOI:

https://doi.org/10.55251/jmbfs.9929

Keywords:

Antimicrobial activity, Antioxidant activity, Fibroblast proliferation, Liposome production, Liposome characterization, Plantago major L. extracts

Abstract

The objectives of this study are to determine the effective features of water and aqueous-ethanol extracts of Plantago major L. (Pm-dH₂O and Pm-EtOH, respectively) in wound healing, such as antimicrobial, antioxidant potential, and fibroblast proliferation. Furthermore, it was targeted to obtain liposome structures loaded with Pm-extract for controlled release, characterize the obtained liposomes, and determine their release profiles. Antimicrobial activity (inhibition zone and minimum inhibition concentration), total antioxidant and oxidant (TAS, TOS, and OSI) potentials, total phenolic content (TPC), and total flavonoid content (TFC) of Pm extracts were tested. The radical scavenging activity of Pm extracts was quantified by the DPPH assay. Antioxidant capabilities of Pm extracts were determined with ABTS, CUPRAC, and FRAP. In vitro cytotoxicity tests of Pm extracts were examined on dermal fibroblast cells with MTT assay. Liposome structures loaded with Pm extracts were obtained using the ethanol injection method. Plant extract-loaded liposomes were characterized, and in vitro release kinetics were revealed by dialysis technique.

Pm-EtOH extract inhibited the growth of microorganisms at lower concentrations than Pm-dH₂O extract. According to TAS, TOS, and OSI values, it was concluded that there was a moderate antioxidant effect in Pm extracts in general. TPC and TFC of the Pm-EtOH extract are higher than the Pm-dH₂O extract. Inhibition percentages of Pm-EtOH extract are higher than Pm-dH₂O extracts against DPPH free radicals. The ABTS, CUPRAC, and FRAP antioxidant capacities of the Pm-EtOH extract are higher than that of the Pm-dH₂O extract. Furthermore, according to dynamic light scattering and microscopy images, these plant extracts loaded liposomes had a high entrapment efficiency, a wide size range, and good stability.

Pm extracts showed high cell viability and no cytotoxic effect on HDF cells. Liposome structures loaded with Pm extracts are fine alternatives for effective and controlled release to the wound site due to high entrapment efficiency, a wide size range, and good stability.