PRECISION AND RELIABILITY OF NANOPLATE DIGITAL PCR SYSTEM FOR PORK DNA IDENTIFICATION AND QUANTIFICATION

Rini Hafzari; Annisa; Anita Kairani; Muchamad Nur Cholis; Listya Puspa Kirana; Nurul Huda Pangabean; Nurbaity Situmorang; Dwi Ratna Anjaning Kusuma Marpaung

doi:10.55251/jmbfs.10691

PRECISION AND RELIABILITY OF NANOPLATE DIGITAL PCR SYSTEM FOR PORK DNA IDENTIFICATION AND QUANTIFICATION

Authors

Rini Hafzari Universitas Negeri Medan https://orcid.org/0000-0001-9809-6482
Annisa Padjadjaran University https://orcid.org/0000-0001-6642-4349
Anita Kairani Medan State University
Muchamad Nur Cholis PT. Genecraftlabs https://orcid.org/0000-0002-5785-4483
Listya Puspa Kirana PT. Genecraftlabs https://orcid.org/0009-0003-6922-8791
Nurul Huda Pangabean Medan State University https://orcid.org/0009-0008-1495-5023
Nurbaity Situmorang Medan State University https://orcid.org/0000-0001-9672-6567
Dwi Ratna Anjaning Kusuma Marpaung Medan State University https://orcid.org/0009-0009-6860-4752

DOI:

https://doi.org/10.55251/jmbfs.10691

Keywords:

Species Identification, Nanoplate Digital PCR, DNA Quantification

Abstract

The adulteration and mislabeling of food ingredients, particularly meat products, pose a significant concern for consumers. In order to address this issue, it is crucial to use precise and sensitive methods for species identification, mainly when dealing with samples that contain a limited number of copies of DNA. The primary objective of this study is to design a digital nanoplate polymerase chain reaction (PCR) technique for the purpose of quantifying species identification. The primer used in this study was cytochrome b gene. The results showed that target DNA can be amplified using the digital PCR nanoplate until the lowest sample concentration of 0.0013 ng/µL. This study can be applied to enhance species identification processes in food samples by utilizing digital PCR nanoplates.

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Published

2024-05-23

How to Cite

Rini Hafzari, Annisa, Anita Kairani, Muchamad Nur Cholis, Listya Puspa Kirana, Nurul Huda Pangabean, Nurbaity Situmorang, & Dwi Ratna Anjaning Kusuma Marpaung. (2024). PRECISION AND RELIABILITY OF NANOPLATE DIGITAL PCR SYSTEM FOR PORK DNA IDENTIFICATION AND QUANTIFICATION. Journal of Microbiology, Biotechnology and Food Sciences, 14(1), e10691. https://doi.org/10.55251/jmbfs.10691

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Issue

Vol. 14 No. 1 (2024): August - September

Section

Food Sciences

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