MOLECULAR IDENTIFICATION USING 16S rRNA GENE TO IDENTIFY BACTERIA SYMBIONT-Agelas sp. SPONGE WITH ANTIBACTERIAL ACTIVITY
DOI:
https://doi.org/10.55251/jmbfs.11840Keywords:
Marine Sponge, Symbiont Bacteria, 16SrRNA, Antibacterial, Bioactive CompoundAbstract
Sponge (Agelas sp.) are complex marine organisms that form symbiotic relationships with bacterial communities. These bacterial symbionts produce bioactive secondary metabolites with various therapeutic potentials, including anticancer, antiviral, anti-inflammatory, and antibacterial effects. This study aims to identify bacterial symbionts associated with Agelas sp. sponge from Sibolga, North Sumatera, that have antibacterial activity, using the 16S rRNA gene. The antibacterial activity of these symbionts was evaluated against Staphylococcus lugdunensis ESBL, Pseudomonas aeruginosa ESBL, and Klebsiella pneumoniae ESBL. Ten bacterial isolates exhibiting significant antibacterial activity were identified. Sequencing gen 16S rRNA result showed that these isolates included Bacillus cereus, Pseudomonas aeruginosa, Alcaligenes, and Vibrio species, demonstrating efficacy against the tested pathogenic bacteria. The results showed the potential of Agelas sp. symbiotic bacteria as a source of natural antibacterial compounds that can be used to develop new antibacterial agents. This study offers a valuable foundation for future exploration of bioactive compounds from sponges with antibacterial applications.
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Copyright (c) 2023 Endang Sulistyarini Gultom, Urip Harahap, Dwi Suryanto, Herbert Sipahutar, Rini Hafzari

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