OPTIMIZATION OF CELLULASE-ASSISTED EXTRACTION PARAMETERS TO ENHANCE THE YIELD OF NUTRITIONAL COMPOUNDS FROM OYSTER MUSHROOMS (PLEUROTUS SAJOR-CAJU)

Abstract

Oyster mushroom (Pleurotus sajor-caju) has substantial potential as a raw material widely utilized in food production. This study aimed to optimize the extraction conditions of dried oyster mushroom material with the assistance of cellulase enzyme. A central composite design was employed, incorporating varying dilution ratios of water to dried mushroom powder (15:1, 20:1, 25:1), enzyme concentrations (0.3, 0.4, 0.5 w/v), and pH levels of 4.5, 5.0, and 5.5. The results showed excellent agreement with quadratic regression models, with R² > 0.88. Based on the response surface plots, the optimal parameters were determined to be a dilution ratio of 1:19.6, a pH of 5, and a cellulase concentration of 0.44% (w/v). Under these optimal conditions, the nutrient content (saccharose, reducing sugar, protein, amino protein) and the levels of bioactive compounds (lysine, β-glucan, phenolics, flavonoids) per 100 grams of dry matter were quantified as 8.24 g, 3.60 g, 3.95 g, 3.10 g, 0.08 g, 2.17 mg, 4.93 g TAE, and 0.65 g QE, respectively.