SEARCHING AND IN SILLICO CHARACTERIZATION OF STREPTOMYCES PHAGE ENDOLYSINS AND THEIR CATALYTIC DOMAINS

Darina Peterkova; Zuzana  Å ramkovÃ¡; Michaela  MancoÅ¡; Andrej  GodÃ¡ny

doi:10.15414/jmbfs.2020.10.2.221-229

SEARCHING AND IN SILLICO CHARACTERIZATION OF STREPTOMYCES PHAGE ENDOLYSINS AND THEIR CATALYTIC DOMAINS

Authors

Darina Peterkova University of Ss. Cyril and Methodius in Trnava, Faculty of Natural Sciences, Department of Biology
Zuzana Å ramkovÃ¡
Michaela MancoÅ¡
Andrej GodÃ¡ny

DOI:

https://doi.org/10.15414/jmbfs.2020.10.2.221-229

Keywords:

Streptomyces spp., endolysin, in sillico, bioinformatic, function domains

Abstract

Endolysins, peptidoglycan hydrolases encoded by bacteriophages, degrade bacterial cell wall and are promising alternative to antibiotics. Howewer, the selection and bioengineering of endolysins needs previous bioinformatic characterization. This study focuses on endolysins encoded by viruses infecting Streptomyces spp., describes Streptomyces phages diversity and association. Set of 143 endolysins were predicted in 175 Streptomyces phage genomes from NCBI database and The Actinobacteriophage Database and were characterized their phylogeny and function domains, especially catalytic domains. Predicted endolysins have amidase (Ami_2 and CHAP domain), muramidase (glycol_hydro_25) and peptidase activity (CHAP, NlpC/P60). This bioinformatic characterization serve as a base to next research in developing of endolysins with new properties in enzybiotik form.

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Published

2020-10-16

How to Cite

Peterkova, D., Å ramkovÃ¡, Z. ., MancoÅ¡, M. ., & GodÃ¡ny, A. . (2020). SEARCHING AND IN SILLICO CHARACTERIZATION OF STREPTOMYCES PHAGE ENDOLYSINS AND THEIR CATALYTIC DOMAINS. Journal of Microbiology, Biotechnology and Food Sciences, 10(2), 221–229. https://doi.org/10.15414/jmbfs.2020.10.2.221-229

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Issue

Vol. 10 No. 2 (2020): October - November

Section

Biotechnology

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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