LYOPHILIZED AQUEOUS EXTRACT OF PINUS HALEPENSIS (MILL.) RESIN: CHEMICAL COMPOSITION, ANTIOXIDANT AND ANTIDERMATOPHYTIC ACTIVITIES

Authors

  • Mohamed Mihoub Zerroug University Ferhat Abbas Setif 1
  • Nora Haichour
  • Samia Mezaache Aichour
  • ElKhamsa Soltani
  • Seousen Kada
  • José Rodríguez Martinez
  • Maria Angeles Esteban
  • Jane Nicklin

DOI:

https://doi.org/10.15414/jmbfs.3423

Keywords:

Pinus halepensis, resin, GC/MS, antioxidant activity, antifungal activity

Abstract

In traditional Algerian medicine, Pinus halepensis (Mill.) resin is used as antimicrobial, anti-inflammatory, analgesic, wound healer, for the treatment of respiratory and urinary diseases. In the present study, a lyophilized aqueous extract (LAE) of Pinus halepensis (Mill.) resin is subjected to chemical composition analysis by gas chromatography coupled to mass spectrometry (GC/MS), antioxidant activity was evaluated in vitro using 2,2-diphenyl-b,picrylhydrazyl (DPPH) and β-carotene bleaching assays. LAE of Pinus halepensis (Mill.) resin was also tested for its potential activity against four dermatophytic fungi: Trichophyton rubrum, Trichophyton equinum, Trichophyton mentagrophytes, Trichophyton tonsurans. Results showed that LAE of Pinus halepensis (Mill.) resin contained 64.57% of aromatic compounds. This extract contains also succinic acid, sugars and alcohol sugars, alkaloids and other compounds that represent respectively 0.58%, 6.45%, 0.87% and 2.05%. The obtained extract exhibited satisfying antioxidant activity with an inhibition percentage of 93.76±0.41 at a concentration of 300 µg/mL and IC50 of 83.64µg/mL by the DPPH method. The tested extract showed also a good antioxidant activity using β-carotene test. At resin concentration of 100 µg/mL antifungal tests showed, inhibition rates of 58.9%, 58.44%, 41.36% and 34.43% against T. tonsurans, T. equinum, T. rubrum and T. mentagrophytes respectively. Upon the obtained results, this extract can be used as external treatments for wounds to prevent oxidation and fungal infections.

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Published

2021-05-18

How to Cite

Zerroug, M. M., Haichour, N. ., Mezaache Aichour, S., Soltani, E., Kada, S., Martinez, J. R., Angeles Esteban, M., & Nicklin, J. (2021). LYOPHILIZED AQUEOUS EXTRACT OF PINUS HALEPENSIS (MILL.) RESIN: CHEMICAL COMPOSITION, ANTIOXIDANT AND ANTIDERMATOPHYTIC ACTIVITIES. Journal of Microbiology, Biotechnology and Food Sciences, e3423. https://doi.org/10.15414/jmbfs.3423

Issue

Section

Biotechnology
Received 2020-07-10
Accepted 2021-05-11
Online Published 2021-05-18