IN VITRO BIOSYNTHESIS AND ANTIMICROBIAL POTENTIAL OF BIOLOGICALLY REDUCED GRAPHENE OXIDE/AG NANOCOMPOSITE AT ROOM TEMPERATURE
Keywords:Reduced graphene oxide, Silver, nanocomposite, Biosynthesis, Antimicrobial resistance
Antimicrobial resistance is one of the most serious problems that continues to challenge the public health threats and agriculture sectors. The study highlighted one-step method for reduced graphene oxide/Ag nanocomposite (rGO/AgNC) biosynthesis using the supernatant of Escherichia coli D8 (MF062579) strain at room temperature and sunlight. The rGO/AgNC was characterized by UV-vis spectrophotometry, Fourier transform-infrared (FT-IR) spectroscopy and transmission electron microscopy (TEM). Results showed those the rGO-annealed AgNPs showed absorption peak at 430 nm and have been obtained with an average particle size of 8-17Â±9.1 nm. The MIC value of rGO/AgNC (6.25 Î¼g/mL) showed in vitro antimicrobial inhibition against pathogenic bacterial strains such as Gram-negative bacteria (E. coli ATCC25922; 79%, Klebsiella pneumoniae ATCC33495; 87%) and Gram-positive bacteria (Staphylococcus aureus ATCC25923; 91% and Bacillus cereus ATCC6633; 53%) as well as Candida albicans ATCC10231; 60% as pathogenic yeast.
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Copyright (c) 2021 Mohamed Marzouk El-Zahed, Zakaria A. M. Baka, M. I. Abou-Dobara, Ahmed K.A. El-Sayed
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