IN VITRO BIOSYNTHESIS AND ANTIMICROBIAL POTENTIAL OF BIOLOGICALLY REDUCED GRAPHENE OXIDE/AG NANOCOMPOSITE AT ROOM TEMPERATURE

Authors

  • Mohamed Marzouk El-Zahed Teaching assistance
  • Zakaria A. M. Baka Professor of microbiology
  • M. I. Abou-Dobara Professor of bacteriology
  • Ahmed K.A. El-Sayed Professor of molecular microbiology

DOI:

https://doi.org/10.15414/jmbfs.3956

Keywords:

Reduced graphene oxide, Silver, nanocomposite, Biosynthesis, Antimicrobial resistance

Abstract

Antimicrobial resistance is one of the most serious problems that continues to challenge the public health threats and agriculture sectors. The study highlighted one-step method for reduced graphene oxide/Ag nanocomposite (rGO/AgNC) biosynthesis using the supernatant of Escherichia coli D8 (MF062579) strain at room temperature and sunlight. The rGO/AgNC was characterized by UV-vis spectrophotometry, Fourier transform-infrared (FT-IR) spectroscopy and transmission electron microscopy (TEM). Results showed those the rGO-annealed AgNPs showed absorption peak at 430 nm and have been obtained with an average particle size of 8-17±9.1 nm. The MIC value of rGO/AgNC (6.25 μg/mL) showed in vitro antimicrobial inhibition against pathogenic bacterial strains such as Gram-negative bacteria (E. coli ATCC25922; 79%, Klebsiella pneumoniae ATCC33495; 87%) and Gram-positive bacteria (Staphylococcus aureus ATCC25923; 91% and Bacillus cereus ATCC6633; 53%) as well as Candida albicans ATCC10231; 60% as pathogenic yeast.

Author Biographies

Zakaria A. M. Baka, Professor of microbiology

Department of Botany and Microbiology, Faculty of Science, Damietta University, New Damietta, Egypt

M. I. Abou-Dobara, Professor of bacteriology

Department of Botany and Microbiology, Faculty of Science, Damietta University, New Damietta, Egypt

Ahmed K.A. El-Sayed, Professor of molecular microbiology

Department of Botany and Microbiology, Faculty of Science, Damietta University, New Damietta, Egypt

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Published

2021-06-01

How to Cite

El-Zahed, M. M., Baka, Z., Abou-Dobara, M. I., & El-Sayed, A. . (2021). IN VITRO BIOSYNTHESIS AND ANTIMICROBIAL POTENTIAL OF BIOLOGICALLY REDUCED GRAPHENE OXIDE/AG NANOCOMPOSITE AT ROOM TEMPERATURE. Journal of Microbiology, Biotechnology and Food Sciences, 10(6), e3956. https://doi.org/10.15414/jmbfs.3956

Issue

Section

Microbiology
Received 2020-11-12
Accepted 2021-01-03
Online Published 2021-06-01