ANTIOXIDATIVE PROPERTIES OF ETHYL ACETATE FRACTION OF UNRIPE PULP OF CARICA PAPAYA IN MICE

Authors

  • Omotade Oloyede
  • Jeferson Franco
  • Daniel Roos
  • Joao Rocha
  • Magareth Athayde
  • Aline Boligon

Keywords:

antioxidant, Carica papaya, quercetin, β-sitosterol, antioxidant enzymes

Abstract

Unripe Carica papaya fruits were extracted with methanol in Soxhlet apparatus and later with a liquid-liquid extraction with the aim of identifying and quantifying secondary metabolite fraction of this plant. Quercetin and β-sitosterol have been isolated from the fruit and the quantities detected were 120.2±0.16 mg/g (dry fruit) and 279.1±0.09mg/g respectively. In addition, the extracts were evaluated in vivo for their effects on activities of some antioxidant enzymes which includes Glutathione peroxidase (GPx), Glutathione reductase (GR), Glutathione transferase (GST), Catalase (CAT) and metabolizing enzyme Glucose-6-phosphate dehydrogenase (G6PDH) in mice treated orally with a daily dose of extracts (100mg/kg) for 7 days. Results showed that ethyl acetate fraction caused significant increase(p<0.05) in the activities of GR, GPx, GST, and G6PDH. Significant decrease (p<0.05) in GPx activity was observed in kidney following administration of ethyl acetate fraction. It is likely that quercetin and β-sitosterol may be responsible for the antioxidant potential demonstrated by the ethyl acetate fraction from unripe fruit.

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Published

2021-03-11

How to Cite

Oloyede, O. ., Franco, J. ., Roos, D. ., Rocha, J. ., Athayde, M. ., & Boligon, A. . (2021). ANTIOXIDATIVE PROPERTIES OF ETHYL ACETATE FRACTION OF UNRIPE PULP OF CARICA PAPAYA IN MICE. Journal of Microbiology, Biotechnology and Food Sciences, 1(3), 409–425. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/4470

Issue

Vol. 1 No. 3 (2011): December - January

Section

Biotechnology

License

Copyright (c) 2011 Omotade Oloyede, Jeferson Franco, Daniel Roos, Joao Rocha, Magareth Athayde, Aline Boligon

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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