THE ACTIVITY OF SUPEROXIDE DISMUTASE AND GLUTATHIONE PEROXIDASE IN KIDNEYS AND LIVER OF MICE EXPOSED TO ACRYLAMIDE

Abstract

Acrylamide monomer causes neurotoxicity, male reproductive toxicity and endocrine – related tumors in rodents. Acrylamide was found in food, heat-processed potato products, coffee, bread and has been classified as probable human carcinogen by IARC. Daily intake from foodstuff was estimated in the range of 0.3-0.8 mg/kg bw per day (Seal et al., 2008). Glutathione peroxidase catalyzes the reduction hydrogen peroxide by glutathione. Extracellular superoxide dismutase catalyzes the dismutation of superoxide radical to hydrogen peroxide and oxygen. The activity of superoxide dismutase and glutathione peroxidase has been examined. The research was conducted on SWISS mice 8 week old, weight 26 g. Decapitation was carried out after 48, 72 and 192 hours after acrylamide injection. Animals received two doses of acrylamide 20 mg/kg and 40 mg/kg with intraperitoneal injection three times a week. Control group received physiological saline 3 times a week. The activity of superoxide dismutase was higher in the kidneys than in the liver. The activity of glutathione peroxidase was reported higher in the kidneys than in the liver. Studies in Drosophila melanogaster have shown that acrylamide causes oxidative stress and clearly enhances reactive oxygen species and interferes of oxidative enzyme activity (Prasad and Muralidhara, 2012).