STABILIZING THE GONADOTROPIN ACTIVITY WITH THE USE OF ORGANIC COMPOUNDS

Authors

  • Yurij Slyvchuk
  • Ivan Hevkan
  • Iryna Matiukha
  • Vasyl Syrvatka

Keywords:

Chorionic Gonadotropin, Stabilization, L-lysine, L-glycine, Immunochemiluminescent assay

Abstract

Complex studies of optimum quantitative and qualitative composition of carbohydrates and amino acids necessary for gonadotropines activity stabilization were the aim of our investigations. This was determined by biological active substances ─ amino acids: L- lysine , L- glycine and carbohydrates: sucrose and mannitol, which stabilize gonadotropins activity for longer use while preserving activity. As a result of the studies we found out that the addition 10 mg/sm3 of L-lysine and 75 mg/sm3 of sucrose for extended incubation provides the highest gonadotropin activity compared with other experimental and control batches of samples. In the experimental series of samples, where the stabilizer was added at a rate of 10 mg/sm3 L-lysine and 75 mg/sm3 mannitol, the activity was stable during 6 weeks of incubation and constituted more than 50% of the initial theoretical activity of hCG.

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Published

2014-02-01

How to Cite

Slyvchuk, Y., Hevkan, I., Matiukha, I., & Syrvatka, V. (2014). STABILIZING THE GONADOTROPIN ACTIVITY WITH THE USE OF ORGANIC COMPOUNDS. Journal of Microbiology, Biotechnology and Food Sciences, 3(special issue 2 (Biotechnology), 160–163. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7645

Issue

Vol. 3 No. special issue 2 (Biotechnology) (2014): February

Section

Biotechnology

License

Copyright (c) 2014 Yurij Slyvchuk, Ivan Hevkan, Iryna Matiukha, Vasyl Syrvatka

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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