ASSESSMENT OF THE ANTIOXIDANT POWER OF IN VITRO OBTAINED COLEUS FORSKOHLII BRIQ
Keywords:Coleus forskohlii Briq., Rooting, Antioxidant activity, Auxin
In thÐµ prÐµsent studÑƒ, a simÑ€le protÐ¾ÑÐ¾l for in vitro rooting induction of the important medicinal plant Coleus forskohlii Briq with a high content of secondary metabolites with antioxidant activity was described. For the in vitro germination seeds of C. forskohlii were placed on MS medium suÑ€Ñ€lemented with 0.4 mg L-1 gibberellic acid and 1.0 mg L-1 CaCl2. The Ð¾btÐ°inÐµd shoots were cultured on a half-strength Â½ MS medium containing indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) at concentrations of 0.1 and 0.5 mg L-1. The maximum rooting of plants (95%) with mean values of 9.2 roots per explant, root length (5.1 cm) and root fresh weight (0.085 g) was obtained on half-strength MS medium with 0.1 mg L-1 IAA after three weeks of culture. The plants were successfully ex vitro adapted with 93.3% survival on the mixture substrate of soil, peat and sand (2:1:1 v/v/v). The antioxidant enzymes Ð°ÑtivitiÐµs (superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase) showed the highest values when IBA was used for C. forskohlii in vitro cultivation. Applied auxins influenced the level of secondary metabolites synthesis. Half strength MS culture medium supplemented with 0.5 mg L-1 IAA caused the highest total phenol and flavonoid content as well as the highest antioxidant activity level (radical scavenging activity and ferric reducing antioxidant power). The results indiÑÐ°te thÐ°t orgÐ°nÐ¾genÐµsis is Ð° very ÑÐ¾mplicated biologiÑÐ°l prÐ¾ÑÐµss involving up and downregulation of plant enzyme and non-enzyme antioxidant protection systems, which plÐ°Ñƒ an imÑ€Ð¾rtant role during the organogenesis of plants.
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Copyright (c) 2022 Geneva Maria, Ira Stancheva, Elisaveta Kirova, Maria Petrova, Saber Hendawy, Ely Zayova
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