ASSESSMENT OF THE EFFECT OF PLANT GROWTH REGULATORS ON IN VITRO MICROPROPAGATION AND METABOLIC PROFILES OF MELISSA OFFICINALIS L. (LEMON BALM)

Authors

  • Maria Petrova Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences https://orcid.org/0000-0002-0750-1364
  • Milena Nikolova
  • Margarita Dimitrova
  • Lyudmila Dimitrova

DOI:

https://doi.org/10.15414/jmbfs.4077

Keywords:

in vitro, multiplication, total phenols, metabolites, GC/MS analysis, medicinal plant

Abstract

In this study the effectiveness of plant growth regulators on micropropagation, total phenol content and metabolic profiles of Melissa officinalis L., important medicinal herb was assessed. The stem segments derived from one-month old in vitro germinated seedlings were used for initial explants. Of the eight different nutrient media tested the most favorable for micropropagation were found to be MP2 (MS medium enriched with 1mg/L BAP and 0.1 mg/L IBA) and MP3 (MS supplemented with 1.5 mg/L BAP and 0.5 mg/L NAA), which yielded 3.25- 4.0 shoots per explant within 4 weeks of culture. In vitro rooting (100%) was achieved on half strength MS medium contained 2% sucrose. Rooted plantlets were adapted successfully (98%) in a mixture of soil, peat, perlite, and sand in ratio 2:1:1:1 (v/v/v/v). Twenty metabolites were identified by GC/MS analysis of shoots grown on the MS media including different types of growth regulators. Analysis revealed the presence of phenolic, organic and fatty acids, sterols, triterpenes, fatty alcohols, saccharides and polyols. The studied samples have the same metabolic profile with quantitative differences in the individual metabolites. The maximum content of total phenols (13.92 mg/g extract) was obtained in shoots grown on MP2 medium and the lowest was recorded in the microplants cultured on MP1 control medium (8.45 mg/g extract). The obtained results indicated that it is possible to achieve the accumulation of desired metabolites by selection of plant growth regulators added in nutrient media.

Downloads

Published

2021-10-01

How to Cite

Petrova, M., Nikolova, M. ., Dimitrova, M. ., & Dimitrova, L. . (2021). ASSESSMENT OF THE EFFECT OF PLANT GROWTH REGULATORS ON IN VITRO MICROPROPAGATION AND METABOLIC PROFILES OF MELISSA OFFICINALIS L. (LEMON BALM). Journal of Microbiology, Biotechnology and Food Sciences, e4077. https://doi.org/10.15414/jmbfs.4077

Issue

Section

Biotechnology
Received 2020-12-13
Accepted 2021-05-19
Online Published 2021-10-01

Most read articles by the same author(s)