ALKALINE CELLULASE FREE XYLANASE FROM BACILLUS SP. ASX42: PROPERTIES, PURIFICATION AND ITS EFFECT ON SEED GERMINATION

Abstract

Bacillus sp. ASX42 isolated from soil samples of Lake Van shores, Turkey, and the strain producing cellulose-free xylanase enzyme with an optimum pH 9.0, at 60ºC. The molecular weight of the enzyme was estimated as 66 kDa on SDS PAGE analysis. Thermal stability of enzyme was detected average 68.7% at 4-60ºC and 60% at 65-95ºC while pH stability was observed about 90% between pH 3.5-13.0 for 15 min. As HgCl₂ presented a strong inhibitor activity, Cobalt (132%) and Manganese (130%) showed a stimulatory effect on xylanase activity. The remaining activity was found to be 77%, 89%, and 92% in the presence of EDTA, 1,10-Phenanthroline monohydrate and β- mercaptoethanol, respectively. In this study, some known purification materials were compared for effectiveness. According to the results, cellulase-free xylanase ASX42 has shown a stimulatory effect on germination of Anagyris foedita and Ceratonia siliqua seeds. It has also produced a whitening effect on wastepaper pulp.