THE IN VITRO INVESTIGATION OF ortho-/meta-/para-ALKOXYPHENYLCARBAMIC ACID ESTERS CONTAINING SUBSTITUTED N-PHENYLPIPERAZIN-1-YL FRAGMENT AGAINST MYCOBACTERIUM TUBERCULOSIS H37Ra STRAIN

Abstract

The purpose of current paper was to in vitro screen the set of ortho-/meta-/para-alkoxyphenylcarbamic acid esters containing 4-(3- trifluoromethylphenyl)piperazin-1-yl moiety for their potency against avirulent Mycobacterium (M.) tuberculosis H37Ra by applying the micromethod for the determination of the minimum inhibitory concentration (MIC). Considered mycobacterial strain was grown in Middlebrook broth, supplemented with Oleic-Albumin-Dextrose-Catalase supplement and mycobactin J (2 µg/mL) as well. The susceptibility of the strain was investigated in a 96-well plate format, the plates were incubated at 37°C for 7 days. According to estimated MIC readouts it was concluded that para-alkoxy substitution within lipophilic fragment would be favorable for the activity of currently investigated compounds against given mycobacterium. On the other hand, relatively high lipophilicity was not regarded as crucial factor which determined the activity profile of inspected derivatives. Moreover, the importance of the lipohydrophilic properties was considered to be the main difference compared to previous conclusions from in vitro screening of these compounds against virulent M. tuberculosis H37Rv.