SCREENING OF EXTRACELLULAR ENZYMATIC ACTIVITY OF MACROFUNGI

Abstract

Six types of extracellular enzymes activities in thirty cultures of Macrofungi (Macromycetes) of different ecophysiological (wood decaying, saprotrophic, entomophilous, and leaf-litter decaying) and taxonomic fungi groups were studied. Amylase activity was detected in all investigated mushroom cultures, lipase – in 26, laccase – 21, urease – 20. Protease activity was revealed in 6 species and nitrate reductase activity only in Lepista luscina and Morchella esculenta. As a whole, L. luscina with its amount of detected enzymes and their good visualization seemed to be a promising species. Some of investigated mushrooms are reported as species producing extracellular enzymes investigated in this study for the first time: Hohenbuehelia myxotricha, L. luscina, Lyophyllum schimeji, Phellinus igniarius, Piptoporus betulinus, and Spongipellis litschaueri (amylase); L. luscina, Crinipellus schevczenkovi, Auriporia aurea, Hypsizygus marmoreus, L. schimeji, Oxyporus obducens, and S. litschaueri (laccase); A. aureus, C. schevczenkovi, H. myxotricha, L. luscina, L. schimeji, O. obducens, and S. litschaueri (lipase); A. aureus, C. schevczenkovi, H. myxotricha, H. marmoreus, L. luscina, L. schimeji, Pleurotus djamor, and S. litschaueri (urease) as well as L. luscina (nitrate reductase).
Characterization of enzymatic activity of individual fungal cultures increases the value of the respective culture collection, and simultaneously makes an easier choice to select fungal strains possessing desired enzymatic potential.