DIFFERENCES IN Na+, K+ , Cl- IONS LEVEL AFTER INTRAPERITONEAL STREPTOZOTOCIN INJECTION IN CHOSEN BRAIN STRUCTURES

Abstract

Na+, K+, Cl- play fundamental role of transport in maintaining the necessary ionic gradients for cell metabolism and excitability in the nervous system.
The aim of our work was to estimate the level of Na+, K+, Cl- ions in selected brain structures after streptozotocin in: the: right hemisphere, the left hemisphere, cerebellum and trunk of the brain. The experiment was carried out on 24 male mice of Swiss strain. The measurements were performed after 48, 72 hours, 8 days and 16 days after streptozotocin injection in single dose – 65 mg/kg b.w. The level of ions was measured in the mixture of supernatant on the EasyLyte Na/K/Cl analyzer. Statistical analysis was performed using analysis of variances ANOVA. Homogoneity of variances was estimates using Dunnett test.
We noticed influence of streptozotocin on ions level in selected brain structures. Taking into account Na+ level there were significant changes in the right hemisphere after 16 days, in the left hemisphere after 16 days, in cerebellum after 48 hours and in trunk of the brain after 16 days. Taking into account K+ level there were significant changes in the right hemisphere after 16 days, in the left hemisphere after 48 hours, 8 and 16 days, in cerebellum after 8 and 16 days and in trunk of the brain after 16 days. Taking into account Cl- level there were significant changes in the right hemisphere after 48 hours, 8 and 16 days, in cerebellum after 48 and 72 hours and in trunk of the brain after 72 hours and 16 days. We can notice many fluctuations in ions level. It was probably the result of the body's attempt to adapt to the inject dose of streptozotocin.
Although no high significant changes after each time, we noticed increased tendency in Na+, K+, Cl- ions level. Significantly increased level of Na+, K+, Cl- ions occures in diabetic patients.