IN VITRO EVALUATION OF PROBIOTIC POTENTIAL OF Lactiplantibacillus plantarum TRG1 ISOLATED FROM AN ALGERIAN’S GOAT RUMEN

Abstract

This present paper is intended to demonstrate the desirable probiotic properties of Lactiplantibacillus plantarum TRG1 isolated from the Algerian goat rumen. The study focused on assessing the strain’s survival rate in stress conditions, namely in phenol 0.4%, bile salts 0.3%, in saliva and the Gastrointestinal tract (GIT) simulated conditions. The adhesion ability of the strain with GIT cells, cell surface properties, the production of EPS and β-galactosidase activity were also analyzed. Besides, biogenic amines production, sensitivity to antibiotics and hemolytic activity were evaluated as safety properties. The results showed that the strain can tolerate phenol 0.4% (13.88 ± 2.54%), bile salts 0.3% (10.91± 0.71%), and displayed good viability in saliva and different parts of the GIT. Viability rates were 99.26 % in saliva, 69.68 % in gastric juice, 37.4 % in the ileum. In the duodenum, 93.09 % of viability was attained after 90 min and 53.73% after 180 min of incubation. Furthermore, the strain displayed good adhesion and acceptable surface properties: hydrophobicity (76.94 ±3.36%), auto-aggregation (29.52% ± 6.95), and co-aggregation with E. coli (15.38 ± 05.43), P. aeruginosa (16.05 ± 2.14) and S. aureus (17.33 ± 2.31). A considerable amount of EPS (942.50 ± 32.78 mg/l) was noticed too. Results also showed that the strain did not present hemolytic activity while it can produce the β-galactosidase enzyme. The strain displaced sensitivity to nitroxolin and tetracycline and is resistant to other antibiotics.