BIOSYNTHESIS AND CHARACTERISTICS OF METABOLITES OF Rhodococcus erythropolis AU-1 STRAIN
Keywords:Rhodococcus erythropolis, trehalose lipids, exopolysaccharides, polyhydroxyalkanoates, thermal analysis, UV-Vis and IR spectroscopy
Practically important metabolites of Rhodococcus erythropolis Au-1 strain were obtained: trehalose lipids, exopolysaccharide and cellular polymer – polyhydroxyalkanoate. The structures of the products were established by the methods of TLC, IR and UV-Vis spectroscopy. The purification of trehalose lipids from crude extract was carried out using silica gel column chromatography, one of the trehalose lipids fractions was identified as a trehalose dimycolate. The presence of (NН) group was detected in the exopolysaccharide. For the first time, a biodegradable polymer was isolated from bacteria cells of the strain R. erythropolis Au-1, іt was identified as the polyhydroxyalkanoate. The physico-chemical properties of the metabolites of the strain R. erythropolis Au-1 were established: the surface tension of trehalose lipids fractions was 30,5-43,2 mN/m; the emulsification activity of the exopolysaccharide solutions (1-10 g/L) with vaseline oil was 42-58%. The differential thermal analysis was used to define starting temperatures of degradation of trehalose dimycolate (130 °C), exopolysaccharide (180 °C) and polyhydroxyalkanoate (164 °C, melting point 42oC). The obtained results expand the possibilities of practical application of trehalose lipids, exopolysaccharide and polyhydroxyalkanoate of the strain R. erythropolis Au-1.
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Copyright (c) 2021 Ihor Semeniuk, Nataliia Koretska, Viktoria Kochubei, Victor Lysyak, Tetyana Pokynbroda, Elena Karpenko, Halyna Midyana
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
Online Published 2021-10-01