BIOSYNTHESIS AND CHARACTERISTICS OF METABOLITES OF Rhodococcus erythropolis AU-1 STRAIN

Authors

  • Ihor Semeniuk
  • Nataliia Koretska Department of Physical Chemistry of Fossil Fuels of L.M. Litvinenko InPOCCС, National Academy of Sciences of Ukraine https://orcid.org/0000-0003-2438-7783
  • Viktoria Kochubei
  • Victor Lysyak
  • Tetyana Pokynbroda
  • Elena Karpenko
  • Halyna Midyana

DOI:

https://doi.org/10.15414/jmbfs.4714

Keywords:

Rhodococcus erythropolis, trehalose lipids, exopolysaccharides, polyhydroxyalkanoates, thermal analysis, UV-Vis and IR spectroscopy

Abstract

Practically important metabolites of Rhodococcus erythropolis Au-1 strain were obtained: trehalose lipids, exopolysaccharide and cellular polymer – polyhydroxyalkanoate. The structures of the products were established by the methods of TLC, IR and UV-Vis spectroscopy. The purification of trehalose lipids from crude extract was carried out using silica gel column chromatography, one of the trehalose lipids fractions was identified as a trehalose dimycolate. The presence of (NН) group was detected in the exopolysaccharide. For the first time, a biodegradable polymer was isolated from bacteria cells of the strain R. erythropolis Au-1, іt was identified as the polyhydroxyalkanoate. The physico-chemical properties of the metabolites of the strain R. erythropolis Au-1 were established: the surface tension of trehalose lipids fractions was 30,5-43,2 mN/m; the emulsification activity of the exopolysaccharide solutions (1-10 g/L) with vaseline oil was 42-58%. The differential thermal analysis was used to define starting temperatures of degradation of trehalose dimycolate (130 °C), exopolysaccharide (180 °C) and polyhydroxyalkanoate (164 °C, melting point 42oC). The obtained results expand the possibilities of practical application of trehalose lipids, exopolysaccharide and polyhydroxyalkanoate of the strain R. erythropolis Au-1.

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Published

2021-10-01

How to Cite

Semeniuk, I. ., Koretska, N., Kochubei, V. ., Lysyak, V. ., Pokynbroda, T. ., Karpenko, E. ., & Midyana, H. . (2021). BIOSYNTHESIS AND CHARACTERISTICS OF METABOLITES OF Rhodococcus erythropolis AU-1 STRAIN. Journal of Microbiology, Biotechnology and Food Sciences, e4714. https://doi.org/10.15414/jmbfs.4714

Issue

Section

Biotechnology
Received 2021-04-27
Accepted 2021-08-11
Online Published 2021-10-01

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