IN VITRO ANTIOXIDANT ACTIVITY OF COMPOSITIONS BASED ON BIOSURFACTANTS AND AMINO-CONTAINING HETEROCYCLIC DERIVATIVES OF 1,4-NAPHTOQUINONE

Abstract

Compositions of rhamnolipids (RL) and trehalosе lipids (TL) with amino-containing heterocyclic derivatives of 1,4-naphthoquinone were obtained for the first time. The acute toxicity of amino-containing derivatives of 1,4-naphthoquinone was determined by in silico method using the GUSAR program. We have studied influence of selected compounds and their rhamnolipid and trehalosе lipids compositions on the processes of lipid peroxidation (LPO) and oxidative modification of proteins (OMP) in rat liver tissues upon initiation of free radical oxidation in vitro. It was determined that 1,4-naphthoquinone derivatives have antioxidant activity. RL and TL have a pro-oxidant effect, increase the content of secondary products of lipoperoxidation compared to the control. They have an antioxidant effect in a composition with 1,4-naphthoquinone derivatives. The lead-compound 2-[(6-(4-fluorophenyl-5-oxo-2,5-dihydro-1,2,4-triazin-3-yl)phenyl)amino]naphthalene-1,4-dione (1) and its complex with rhamnolipid show high antioxidant activity (which is confirmed by the decrease in the content of carbonyl groups in the side chains of proteins compared to the control).