THE EFFECT OF RESISTIN ON CRP SECRETION FROM LYMPHOCYTES IN RESPONSE TO INFLAMMATION

Abstract

Resisitin, a cytokine which is produced by adipose tissue, takes a part in decreasing insulin sensitivity, endothelial dysfunc¬tion, and atherosclerosis. Clinical studies confirmed also that resistin is a molecule with a pro-inflammatory potential.
The aim of this study was to examine the influence of resistin supplementation on
C-reactive protein lymphocytic secretion in piglets during different inflammation states: acute induced by injection of streptozotocin (STZ) and chronic associated with obesity. Experiment was carried out on 10 weeks-old piglets (Polish Landrace, females, n=24) kept in standard conditions. Animals were divided into 4 groups: I – control, II – with obesity (chronic inflammation), III – acute inflammation and IV – with obesity and acute inflammation. Peripheral blood lymphocytes (PBL) were isolated from fresh heparinized blood using standard gradient sedimentation technique. Isolated lymphocytes were placed in medium (RPMI 1649, 10% fetal calf serum, L-glutamine, penicillin, streptomycin) and cultured for 72 hours with or without 1mM of resistin. C-reactive protein level was estimated by the
enyme-linked immunosorbent assay (ELISA, Life Diagnostic) in culture supernatant.
The obtained results showed that during acute inflammation and obesity CRP secretion level increased by 290.9% and 193.9% compare to the control group (184.65±15 ng/10 leucocytes, p<0.05), respectively. The addition of resistin enhanced immune response in obese piglets by increasing CRP secretion ( 29.2%; p<0.05).
Based on the results we can confirm that CRP lymphocytic secretion depends on physiological conditions and resistin takes a part in the development of inflammation as
a modulator of the immune system response during metabolic disorders.