INHIBITION OF mTOR KINASE AFFECTS ASTROCYTES GFAP mRNA EXPRESSION IN IN VITRO CONDITIONS

Authors

  • Anna Latacz
  • Krystyna PierzchaÅ‚a-Koziec
  • Joanna Zubel-Łojek
  • Ewa OcÅ‚oÅ„

Keywords:

mTOR, astrocytes, GFAP mRNA expression, rapamycin

Abstract

In this in vitro study the effects of rapamycin – mTOR kinase inhibitor, on the activity of kinase complexes componenents Rictor and Raptor, and on astrocytes activity were investigated. Astrocytes were incubated for 24 hours in various glucose conditions: normoglycemia (CONTROL : 4.5 g glucose.L-1), high glucose (H1: 9 g glucose.L-1 ), very high glucose (H2:13.5g glucose.L-1). Two concentrations of rapamycin (1nM and 10nM) were added to the culture medium. The activity of kinase complexes was evaluated by quantitative PCR measurements of mRNA expressions for Raptor, Rictor and markers of astrocytes activity – GFAP. Significant differences have been detected between control and experimental groups. Observed data suggested that rapamycin reduced mTOR kinase activity in control group and increased activity of astrocytes in elevated glucose conditions in vitro.

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Published

2014-02-01

How to Cite

Latacz, A., PierzchaÅ‚a-Koziec, K., Zubel-Łojek, J., & OcÅ‚oÅ„, E. (2014). INHIBITION OF mTOR KINASE AFFECTS ASTROCYTES GFAP mRNA EXPRESSION IN IN VITRO CONDITIONS. Journal of Microbiology, Biotechnology and Food Sciences, 3(special issue 2 (Biotechnology), 119–121. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7636

Issue

Vol. 3 No. special issue 2 (Biotechnology) (2014): February

Section

Biotechnology

License

Copyright (c) 2014 Anna Latacz, Krystyna Pierzchała-Koziec, Joanna Zubel-Łojek, Ewa Ocłoń

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

All papers published in the Journal of Microbiology, Biotechnology and Food Sciences  are published under a CC-BY licence (CC-BY 4.0). Published materials can be shared (copy and redistribute the material in any medium or format) and adapted (remix, transform, and build upon the material for any purpose, even commercially) with specifying the author(s).