CONVERSION OF WHEAT STRAW INTO FERMENTABLE SUGARS USING CARBOXYMETHYL CELLULASE FROM TRICHODERMA VIRIDE THROUGH BOX-BEHNKEN DESIGN AND ARTIFICIAL NEURAL NETWORK

Authors

  • Rubina Nelofer Food and Biotechnology Research Center (FBRC), PCSIR Laboratories Complex, Ferozpur Road, Lahore 54600, Pakistan
  • Muhammad Nadeem Food and Biotechnology Research Center (FBRC), PCSIR Laboratories Complex, Ferozpur Road, Lahore 54600, Pakistan
  • Muhammad Irfan University of Sargodha
  • Quratulain Syed Food and Biotechnology Research Center (FBRC), PCSIR Laboratories Complex, Ferozpur Road, Lahore 54600, Pakistan
  • Sara Nawaz Biotechnology Department, Lahore College for Women University, Lahore, Pakistan
  • Arifa Tahir Biotechnology Department, Lahore College for Women University, Lahore, Pakistan

DOI:

https://doi.org/10.15414/jmbfs.2021.10.4.626-630

Keywords:

Wheat straw, Trichoderma viride, Cellulase, Characterization, Response surface methodology, Artificial neural network

Abstract

In this study, carboxymethyl cellulase was produced in submerged fermentation characterized and saccharification was optimized through Box-Behnken design. The optima pH and temperature of enzyme produced by Trichoderma viride were 5 and 50 oC, respectively. The crude enzyme had Km and Vmax values of 1.5143 µM and 0.9253µM/min, respectively, using carboxymethyl cellulose as substrate respectively. Three variables including pH (X1), incubation temperature (X2) and substrate concentration (X3) with three levels were used to optimize saccharification of wheat straw having 83% cellulose content using Box Behnken design and Artificial Neural Network (ANN). Results reveal that the proposed model was significant and quadratic effect of these parameters significantly affects the sugar production. Maximum sugar production (28.87 mg/ml) was predicted at RSM predicted levels of pH (6.6), incubation temperature (50 ºC) and (6.2%) substrate concentration, while the levels predicted for pH, temperature and substrate concentration were 5, 50 oC and 3.5 %, respectively, by ANN. The predicted sugar concentration at these levels was 30.72 mg/mL. The observed values at the predicted levels of RSM and ANN were 25.52 and 29.95 mg/mL respectively.

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Published

2021-02-01

Issue

Section

Food Sciences