EFFECTS OF WHOLE FRUIT EXTRACT OF ELAEAGNUS ANGUSTIFOLIA L. ON GLIOBLASTOMA CELL LINES

Authors

  • Samaneh Arab Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran
  • Marjan Bahraminasab Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran
  • Alireza Yazdani Student Research Committee, Semnan University of Medical Sciences, Semnan, Iran
  • Anna Abdolshahi Food Safety Research Center (salt), Semnan University of Medical Sciences, Semnan, Iran

DOI:

https://doi.org/10.55251/jmbfs.4314

Keywords:

Elaeagnus angustifolia L., Glioblastoma, Cell line, Cytotoxicity, Wound-healing-assay, Colony-forming units' assay

Abstract

Elaeagnus. angustifolia L. (EA) is a natural plant food with therapeutic and medicinal properties for treating various diseases such as rheumatoid arthritis and osteoporosis. Since herbal agents are origins of bioactive compounds, they can be applied in complementary treatment protocols. In this study, EA whole fruit extract's cytotoxic effects were investigated on both animal and human glioblastoma cancer cell lines. The extract of whole fruit of EA was obtained by maceration in ethanol. The phytochemical compounds were identified using high-performance liquid chromatography (HPLC). The MTT and LDH assays were performed to evaluate the cytotoxicity effect of the extract and the determination of cell death. Wound-healing assay and colony formation analysis were employed for migration and proliferation evaluation. Based on HPLC analysis, the main flavonoid components of the extract were included rutin and apigenin. The results demonstrated that EA extract at the dose of 125 to 2000 µg/ml and 61.5 to 2000 µg/ml inhibited C6 and U87 cells' viability and induced significant cell cytotoxicity at both 48 and 72 h incubation times. Besides, EA extract significantly inhibited cell migration and colony formation at 250 to 1000 µg/ml concentration. Overall, the results showed that EA extract could inhibit several stages in glioblastoma carcinogenesis in vitro. Therefore, it can be suggested as an anticancer in the clinical treatment approaches of glioblastoma cancer.

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Published

2022-04-01

How to Cite

Arab, S. ., Bahraminasab, M. ., Yazdani, A. ., & Abdolshahi, A. (2022). EFFECTS OF WHOLE FRUIT EXTRACT OF ELAEAGNUS ANGUSTIFOLIA L. ON GLIOBLASTOMA CELL LINES. Journal of Microbiology, Biotechnology and Food Sciences, 11(5), e4314. https://doi.org/10.55251/jmbfs.4314

Issue

Section

Biotechnology
Received 2021-02-07
Accepted 2021-05-19
Online Published 2022-04-01