ANALYSE OF RESTRICTION SITES OF AMARANTH GBSSI GENE IN MUTANT LINES

Abstract

The aim of the study was to identify and detect possible changes of GBSSI gene in 9 amaranth mutant lines and their 2 control samples. Polymerase chain reaction and restriction digest was used to identify GBSSI gene in Amaranthus cruenthus L. genotype Ficha and hybrid K-433 (Amaranthus hypochondriacus L. × Amaranthus hybridus L.). The presence of GBSSI gene was confirmes in all genotypes. Four parts of GBSSI gene was amplified in PCR. Detection of polymorphisms in PciI restriction site was realized. The presence of the restriction site 5´ A*CATGT 3´ was confirmed in all samples. Restriction endonuclease PciI digested the GBSSI gene in GBSSI 1 sequence in all samples equally. Two fragments with 1171 bp and 361 bp were obtained from restriction digest. Specific part GBSSI 3 of GBSSI gene was digested in all amaranth samples into two fragments with size 896 bp and 202 bp.