MARKER PROFILING OF WHEAT WITH DIFFERENT DROUGHT TOLERANCE BY CDDP

Abstract

Wheat is considered to be the world oldest and most widely grown plant. Current genetic and genomic information about wheat is extensive and contains partial knowledge of various types of markers up to the genomic sequence data. Up to date, several different types of DNA markers have been used in the mapping of the wheat genome, that were used to describe the whole genome polymorphism of various wheat varieties. In study, CDDP markers were used to analyse the length polymorphism in the genome of two drought tolerant wheat varieties and two drought susceptible wheat varieties. Polymorphism has not been obtained in relation to the type of drought resistance in the analyzed wheat varieties in the summary of the CDDP profiles. The primer combination WRKY-F and WRKY-R1 has one fragment with a constant amplicon length of 129 bp in all the analyzed varieties. For the combination of WRKY-F and WRKY-R2, the length of the amplicon shifted to 129-130 bp and to the 130-132 bp by F/R3 combination. The combinations of WRKY-F and WRKY-R2b and WRKY-F and WRKY-R3b with the exception of the Dagmar variety (WRKY-F and WRKY-2b) did not amplify amplicons of the given size. For the F/R1 primer combination, the fragments 301-304 bp, 138-140 bp and 187-192 bp were also typical. The second primer combination (WRKY-F and WRKY-R2) has generated fragments of the length of 156-158 bp and 181-186 bp in addition to the 129-130 bp fragment in all varieties. All are nonspecific. The marker system provides monomorphic profiles in all analyzed varieties and the total number of detected fragments was 81.