UTILIZATION OF STORAGE PROTEINS POLYMORPHISM FOR DIFFERENTIATION OF WHEAT, RYE AND TRITICALE GENOTYPES

Abstract

The aim of the paper was to observe and to detect genetic variability of 32 bread wheat genotypes (TriticumaestivumL.), 5 rye genotypes (SecalecerealeL.) and 5 genotypes of triticale (TriticosecaleWittmack.) on the basis of polymorphism of storage proteins. Storage proteins consist of two fractions, which are the main part of grain proteins and are used as a marker not only for genetic variability investigation, but also for characterization of genotypes. Polyacrylamide gel electrophoresis with sodium dodecyl sulphate (SDS-PAGE) was used to separate proteins according their molecular weight. Doc-It LS software was used to detect and to calculate variability of genotypes within individual cereal species. The Jacquard coefficient of similarity and the Unweighted Pair Group Method with Arithmetic Averages (UPGMA) algorithm was used for construction of dendrogram of similarity. Our results show, that genotypes of bread wheat are homogenous and single line, but rye and triticale genotypes were heterogenous. There were observed two main clusters and four subclusters in collection of bread wheat and rye genotypes. Analysis of triticale genotypes similarity show two mail clusters, but only one of them was further separated into two subclusters. There were also recorded, that some of the genotypes have no dissimilarity and is necessary to perform additional analysis using2-D electrophoresis or mass spectrometry. Genotypes PS 28/08 (wheat), Židlochovickéranné (rye) and Benetto (triticale) were the most different.