THE EFFECT OF HT-2 TOXIN WITH YUCCA (YUCCA SHIDIGERA) ON SECRETION OF STEROID HORMONES BY PORCINE OVARIAN GRANULOSA CELLS

Authors

  • Nora Maruniakova
  • Attila Kádasi
  • Alexander V. Sirotkin
  • Jozef Bulla
  • Roland Grossmann
  • Adriana Kolesárová

Keywords:

HT-2 toxin, yucca, progesterone, testosterone, estradiol, granulosa cells

Abstract

The aim of this in vitro study was to examine the secretion of progesterone, testosterone and estradiol by ovarian granulosa cells after addition of HT-2 toxin in combination with yucca. Ovarian granulosa cells were incubated withouth (control) or with treatments at the doses 1, 5 and 50 ng.ml-1 of HT-2 toxin plus yucca 50 µg.ml-1 for 48h. Progesterone, testosterone and estradiol were determined by RIA. After addition of 5, 50 ng.ml-1 (but not at 1 ng.ml-1) of HT-2 toxin with yucca 50 µg.ml-1 the release of progesterone was significantly (P < 0.05) inhibited. The release of testosterone was significantly (P < 0.05) inhibited by 1 ng.ml-1 (but not at 5 and 50 ng.ml-1) of HT-2 toxin with 50 µg.ml-1 of yucca. We observed significant (P < 0.05) stimulation in estradiol release in all experimental groups. There is a little information about the effect of A-trichothecenes on secretion activity of animal ovarian granulosa cells. The results of our in vitro experiments indicate the possible dose-dependent effect of HT-2 toxin with yucca on secretion activity to release steroid hormones.

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Published

2013-06-06

How to Cite

Maruniakova, N., Kádasi, A., V. Sirotkin, A., Bulla, J., Grossmann, R., & Kolesárová, A. (2013). THE EFFECT OF HT-2 TOXIN WITH YUCCA (YUCCA SHIDIGERA) ON SECRETION OF STEROID HORMONES BY PORCINE OVARIAN GRANULOSA CELLS. Journal of Microbiology, Biotechnology and Food Sciences, 2(Abstracts special issue), 16–17. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7544

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