THE EFFECT OF HT-2 TOXIN WITH YUCCA (YUCCA SHIDIGERA) ON SECRETION OF STEROID HORMONES BY PORCINE OVARIAN GRANULOSA CELLS

Abstract

The aim of this in vitro study was to examine the secretion of progesterone, testosterone and estradiol by ovarian granulosa cells after addition of HT-2 toxin in combination with yucca. Ovarian granulosa cells were incubated withouth (control) or with treatments at the doses 1, 5 and 50 ng.ml-1 of HT-2 toxin plus yucca 50 µg.ml-1 for 48h. Progesterone, testosterone and estradiol were determined by RIA. After addition of 5, 50 ng.ml-1 (but not at 1 ng.ml-1) of HT-2 toxin with yucca 50 µg.ml-1 the release of progesterone was significantly (P < 0.05) inhibited. The release of testosterone was significantly (P < 0.05) inhibited by 1 ng.ml-1 (but not at 5 and 50 ng.ml-1) of HT-2 toxin with 50 µg.ml-1 of yucca. We observed significant (P < 0.05) stimulation in estradiol release in all experimental groups. There is a little information about the effect of A-trichothecenes on secretion activity of animal ovarian granulosa cells. The results of our in vitro experiments indicate the possible dose-dependent effect of HT-2 toxin with yucca on secretion activity to release steroid hormones.