GENETIC DIVERSITY ANALYSIS OF COMMON BEAN (PHASEOLUS VULGARIS L.) GENOTYPES USING SCOT POLYMORPHISM

Authors

  • Zuzana Hromadova Slovak University of Agriculture in Nitra; Faculty of Biotechnology and Food Sciences; Institute of Biotechnology; Tr. A. Hlinku 2; 94976 Nitra https://orcid.org/0000-0002-3718-3440
  • Lucia Mikolasova
  • Zelmi­ra Balazova
  • Martin Vivodi­k
  • Milan Chnapek
  • Zdenka Galova

DOI:

https://doi.org/10.55251/jmbfs.5919

Keywords:

common bean, dendrogram, genetic variability, gene specific markers

Abstract

Common bean (Phaseolus vulgaris L.) is legume crop of worldwide importance and due to optimal content of proteins and other essential compounds it has a potential as a functional food. Genetic diversity studies are significant from the point of obtaining information important for breeding process. The goal of present work was to analyze genetic diversity among 34 genotypes of common bean from different countries using 5 SCoT (Start Codon Targeted) markers. Altogether 82 DNA fragments were amplified, out of which 66 fragments were polymorphic with an average of 11 polymorphic fragments per primer. The highest number of polymorphic fragments was detected by marker SCoT 59 (15). The percentage of polymorphism ranged from 57.17% (SCoT 2) to 78.57% (SCoT 19) with an average of 67.3%. PIC values varied from 0.719 (SCoT 19) to 0.886 (SCoT 3) and average PIC value was 0.802. The DI values varied between 0.722 (SCoT 19) and 0.888 (SCoT3), with an average of 0.804. The dendrogram of 34 genotypes of common bean, constructed based on hierarchical cluster analysis separated genotypes into two clusters (I and II). Cluster I was formed by two subclusters. Thirty-three genotypes were included in cluster I and genotype Albena (Slovak Republic), which significantly differed from other genotypes in seed size was included in cluster II. Obtained results support the effectiveness of SCoT markers in the analysis of common bean useful for genotypes differentiating and assessment of genetic diversity in the set of common bean germplasm.

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Published

2022-08-01

How to Cite

Hromadova, Z., Mikolasova, L. ., Balazova, Z. ., Vivodi­k, M. ., Chnapek, M. ., & Galova, Z. . (2022). GENETIC DIVERSITY ANALYSIS OF COMMON BEAN (PHASEOLUS VULGARIS L.) GENOTYPES USING SCOT POLYMORPHISM. Journal of Microbiology, Biotechnology and Food Sciences, 12(1), e5919. https://doi.org/10.55251/jmbfs.5919

Issue

Section

Biotechnology
Received 2022-03-02
Accepted 2022-06-06
Online Published 2022-08-01

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