MOLECULAR CHARACTERIZATION AND GENETIC DIVERSITY STUDIE OF SOYBEAN (GLYCINE MAX L.) CULTIVARS USING RAPD MARKERS

Authors

DOI:

https://doi.org/10.55251/jmbfs.9219

Keywords:

soybean, RAPD, dendrogram, polymorphism, UPGMA

Abstract

Soybean (Glycine max L.) is an important crop plant which contains a high amount of oil and protein. The main goal of the work was to use 13 RAPD (Random Amplified Polymorphic DNA) markers to study genetic polymorphism in a set of 28 soybean genotypes and to construct a dendrogram from the obtained results, based on which we will recommend genotypes for further breeding. In total, using 13 RAPD markers, we determined 108 fragments in a set of 28 soybean genotypes with an average number of 8.31 fragments per genotype. The number of fragments varied from 6 (OPB-08, OPE-07) to 12 (SIGMA-D-01). Of the total number of 108 fragments, 70 were polymorphic with an average number of 5.38 polymorphic fragments per genotype. The number of polymorphic fragments varied from 3 (OPE-07, OPF-14) to 9 (SIGMA-D-01). The average percentage of fragment polymorphism was 63.68% and ranged from 42.86% (OPF-14) to 83.33% (OPB-08). Diversity index (DI) values ranged from 0.710 (OPA-03) to 0.846 (OPD-08) with an average value of 0.763. A dendrogram prepared based on hierarchical cluster analysis using UPGMA algorithm separated 28 soybean genotypes into four clusters. The applied RAPD markers proved to be a suitable tool for detection of the genetic diversity of soybean genotypes. Based on the constructed dendrogram, it is possible to select suitable varieties for marker-assisted breeding.

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Published

2022-10-25

How to Cite

Vivodik, M., Balazova, Z. ., Chnapek, M. ., Hromadova, Z. ., Mikolasova, L. ., & Galova, Z. . (2022). MOLECULAR CHARACTERIZATION AND GENETIC DIVERSITY STUDIE OF SOYBEAN (GLYCINE MAX L.) CULTIVARS USING RAPD MARKERS. Journal of Microbiology, Biotechnology and Food Sciences, e9219. https://doi.org/10.55251/jmbfs.9219

Issue

Section

Biotechnology

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