FAGOPYRUM SPP FINGERPRINT VARIABILITY USING THE ALLERGEN CODING SEQUENCES

Abstract

Buckwheat is one of the plant that possess a high nutrient benefits when used as human food. Up to now, different DNA markers were used to describe intra- and interspecie variability of this plants. Here, BBAP (Bet v 1 amplified polymorphism), PBAP (Profilin based amplified polymorphism) and VBAP (Vicilin based amplified polymor-phism) methods were applied to analyse their ability to distinguish the varieties and genotypes of Fagopyrum esculentum Moench and Fagopyrum tataricum Gaertn. For all of them, polymorphic profiles were obtained with diversity index ranged from 0.87 u to the 0.91. The ability of the primers to distinguish among the analysed varieties and genotypes was highest for BBAP. None of the applied marker techniques was specific for analysed buckwheat species.