RELEASE OF PROGESTERONE AND TESTOSTERONE BY OVARIAN GRANULOSA CELLS AFTER ADDITION OF T-2 TOXIN AND ITS COMBINATION WITH GROWTH FACTOR IGF-I

Abstract

The aim of the present study was to examine the effect of T-2 toxin and combination of this toxin with growth factor IGF-I on secretion of ovarian hormones progesterone P4 and testosterone by ovarian granulosa cells (GCs) of gilts. Ovarian granulosa cells were incubated withouth (control) or with treatments at various doses for 48h: T-2 toxin (10, 100 and 1000 ng.ml-1) / T-2 toxin (10,100 and 1000 ng.ml-1) plus IGF-I (100 ng.ml-1). Progesterone and testosterone were determined by RIA. Progesterone release by GCs was significantly (P<0.05) inhibited after addition of T-2 toxin at all doses 10, 100, 1000 ng.ml-1. Release of testosterone was inhibited after addition of T-2 toxin at 10 and 100 ng.ml-1. On the other hand significant (P<0.05) stimulation of testosterone release at the highest dose 1000 ng.ml-1 was observed. T-2 toxin in combination with growth factor IGF-I inhibited significantly (P<0.05) progesterone release by GCs at all used doses 10, 100, 1000 ng.ml-1of T-2 toxin with 100 ng.ml-1 of IGF-I. Testosterone release was significantly (P<0.05) inhibited after addition of doses 100, 1000 ng.ml-1 of T-2 toxin with 100 ng.ml-1of IGF-I. Our in vitro results examined the dose-dependent effect of T-2 toxin and its combination with growth factor IGF-I on release of progesterone and testosterone by ovarian granulosa cells.