IN VITRO GASTRO – INTESTINAL DIGESTION OF WHEAT COELIAC ACTIVE PROTEINS

Authors

  • Zdenka Gálová
  • Eva Palenčárová
  • Milan Chňapek
  • Želmíra Balážová

Keywords:

gastro-intestinal digestion, coeliac disease, gluten, SDS-PAGE

Abstract

In vitro gastro-duodenal digestion of wheat coeliac active proteins was examined. Three sets of samples of wheat variety Brea were prepared from gastric and another three from duodenal digested samples. Some of proteins were found to be completely resistant to hydrolysis throughout the simulated gastric digestion and some of them throughout the simulated duodenal digestion. The most of polypeptides during subsequent incubation with enzymes resulted in gradual digestion, with only a trace of the parent proteins remaining after gastric digestion, whose majority disappeared after complete gastro-duodenal digestion. Despite the fact that the most of proteins were broken down by gastro-duodenal digestion into low molecular weight peptides, these molecules retain an allergenic potential.

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Published

2012-02-01

How to Cite

Gálová, Z., Palenčárová, E., Chňapek, M., & Balážová, Želmíra. (2012). IN VITRO GASTRO – INTESTINAL DIGESTION OF WHEAT COELIAC ACTIVE PROTEINS . Journal of Microbiology, Biotechnology and Food Sciences, 1(Special issue), 601–609. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7388

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