MICROSATELLITE ANALYSIS OF THE SLOVAK CARNIOLAN HONEY BEE (APIS MELLIFERA CARNICA)

Authors

  • Dušan Paál
  • Ján Kopernick
  • Jaroslav Gasper
  • Dušan Vašíček
  • Katarína Vašíčková
  • Mária Bauerová
  • Miroslav Bauer

Keywords:

carniolan honey bee, microsatellite markers, multiplex PCR

Abstract

The aim of this study was the selection and testing of suitable microsatellite markers for evaluation of the Slovak carniolan honey bee, particularly the population structure, genetic diversity, breed assignment and paternity testing of honey bee queens in Slovakia. Fourteen microsatellite markers running in two multiplex PCR reactions have been tested on 40 randomly selected workers and queens and further verified by PIC index, expected heterozygosity (HE) and observed heterozygosity (HO). Chi-squared test of goodness of fit (α = 0,05) was used to check the Hardy-Weinberg equilibrium (HWE) of genotype for each marker. For the comparison tests the workers of A. mellifera mellifera x ligurica, A. mellifera macedonica and A. mellifera iberica were analyzed, using the same set of markers. We identified a total of 123 alleles in the Slovak carniolan honey bee samples, with the mean value of 8,78 allele per locus. Eleven markers showed the PIC value greater than 0,5 and thus were highly informative. The mean value of expected heterozygosity HE for all loci was 0,705 ± 0,15, the mean value of observed heterozygosity HO was 0,704 ± 0,18. The frequencies of genotypes for most tested markers were in The aim of this study was the selection and testing of suitable microsatellite markers for evaluation of the Slovak carniolan honey bee, particularly the population structure, genetic diversity, breed assignment and paternity testing of honey bee queens in Slovakia. Fourteen microsatellite markers running in two multiplex PCR reactions have been tested on 40 randomly selected workers and queens and further verified by PIC index, expected heterozygosity (HE) and observed heterozygosity (HO). Chi-squared test of goodness of fit (α = 0,05) was used to check the Hardy-Weinberg equilibrium (HWE) of genotype for each marker. For the comparison tests the workers of A. mellifera mellifera x ligurica, A. mellifera macedonica and A. mellifera iberica were analyzed, using the same set of markers. We identified a total of 123 alleles in the Slovak carniolan honey bee samples, with the mean value of 8,78 allele per locus. Eleven markers showed the PIC value greater than 0,5 and thus were highly informative. The mean value of expected heterozygosity HE for all loci was 0,705 ± 0,15, the mean value of observed heterozygosity HO was 0,704 ± 0,18. The frequencies of genotypes for most tested markers were in HWE.

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Published

2013-02-01

How to Cite

Paál, D., Kopernick, J., Gasper, J., Vašíček, D., Vašíčková, K., Bauerová, M., & Bauer, M. (2013). MICROSATELLITE ANALYSIS OF THE SLOVAK CARNIOLAN HONEY BEE (APIS MELLIFERA CARNICA). Journal of Microbiology, Biotechnology and Food Sciences, 2(special issue 2), 1517–1525. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7482

Issue

Vol. 2 No. special issue 2 (2013): Special issue 1 - part A

Section

Biotechnology

License

Copyright (c) 2013 DuÅ¡an Paál, Ján Kopernick, Jaroslav Gasper, DuÅ¡an Vašíček, Katarína Vašíčková, Mária Bauerová, Miroslav Bauer

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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