IN SILICO CHARACTERIZATION OF Β-1,3-GLUCANASE PROMOTER FROM DROSERA BINATA

Abstract

The promoter region of the β-1,3-glucanase gene (DbGluc) was isolated from carnivorous plant species Drosera binata and sequenced. Subsequently, a DNA fragment of 1154 bp upstream of the transcription start site was searched for the presence of motifs identical to cis-element motifs previously reported in the PLACE database. The analysis revealed that the DbGluc promoter contains a TATA-box sequence 35 nucleotides upstream of the transcription start site. Moreover, three functional groups of light-, tissue- and stress-responsive putative cis-elements were identified within the investigated DNA sequence. The group of stress-responsive elements (MYBCORE, ACGTATATERD1, CURECORECR, WRKY-box, WBOXATNPR1, GT1) is very characteristic for promoters of genes encoding pathogenesis-related proteins, including β-1,3-glucanases. These hydrolases may play a role not only in reaction to biotic and abiotic stress but also during the digestive processes in carnivorous species.