IN SILICO CHARACTERIZATION OF Β-1,3-GLUCANASE PROMOTER FROM DROSERA BINATA

Authors

  • Jana Libantova Institute of Plant Genetics and Biotechnology, Plant Science Biodiversity Centre, SAS
  • Monika Frátrikova
  • Martin Jopcik
  • Miroslav Bauer
  • Miroslav Rajninec

DOI:

https://doi.org/10.15414/jmbfs.2670

Keywords:

β-1,3-glucanase, cis-acting elements, Drosera binata, in silico analysis, proximal promoter, transcriptional regulation

Abstract

The promoter region of the β-1,3-glucanase gene (DbGluc) was isolated from carnivorous plant species Drosera binata and sequenced. Subsequently, a DNA fragment of 1154 bp upstream of the transcription start site was searched for the presence of motifs identical to cis-element motifs previously reported in the PLACE database. The analysis revealed that the DbGluc promoter contains a TATA-box sequence 35 nucleotides upstream of the transcription start site. Moreover, three functional groups of light-, tissue- and stress-responsive putative cis-elements were identified within the investigated DNA sequence. The group of stress-responsive elements (MYBCORE, ACGTATATERD1, CURECORECR, WRKY-box, WBOXATNPR1, GT1) is very characteristic for promoters of genes encoding pathogenesis-related proteins, including β-1,3-glucanases. These hydrolases may play a role not only in reaction to biotic and abiotic stress but also during the digestive processes in carnivorous species.

Author Biography

Jana Libantova, Institute of Plant Genetics and Biotechnology, Plant Science Biodiversity Centre, SAS

Department of Molecular Biology and Biotechnology

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Published

2021-10-01

How to Cite

Libantova, J., Frátrikova, M., Jopcik, M., Bauer, M., & Rajninec, M. (2021). IN SILICO CHARACTERIZATION OF Β-1,3-GLUCANASE PROMOTER FROM DROSERA BINATA. Journal of Microbiology, Biotechnology and Food Sciences, e2670. https://doi.org/10.15414/jmbfs.2670

Issue

Section

Biotechnology
Received 2020-02-20
Accepted 2020-06-02
Online Published 2021-10-01