IN SILICO CHARACTERIZATION OF Β-1,3-GLUCANASE PROMOTER FROM DROSERA BINATA
Keywords:β-1,3-glucanase, cis-acting elements, Drosera binata, in silico analysis, proximal promoter, transcriptional regulation
The promoter region of the β-1,3-glucanase gene (DbGluc) was isolated from carnivorous plant species Drosera binata and sequenced. Subsequently, a DNA fragment of 1154 bp upstream of the transcription start site was searched for the presence of motifs identical to cis-element motifs previously reported in the PLACE database. The analysis revealed that the DbGluc promoter contains a TATA-box sequence 35 nucleotides upstream of the transcription start site. Moreover, three functional groups of light-, tissue- and stress-responsive putative cis-elements were identified within the investigated DNA sequence. The group of stress-responsive elements (MYBCORE, ACGTATATERD1, CURECORECR, WRKY-box, WBOXATNPR1, GT1) is very characteristic for promoters of genes encoding pathogenesis-related proteins, including β-1,3-glucanases. These hydrolases may play a role not only in reaction to biotic and abiotic stress but also during the digestive processes in carnivorous species.
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Copyright (c) 2021 Jana Libantova, Monika Frátrikova, Martin Jopcik, Miroslav Bauer, Miroslav Rajninec
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
Online Published 2021-10-01