ADVANCING PROTOCOLS FOR POPLARS in vitro PROPAGATION, REGENERATION AND SELECTION OF TRANSFORMANTS

Authors

  • Nataliia Kutsokon
  • Jana Libantova
  • Volodymyr Rudas
  • Namik Rashydov
  • Dmytro Grodzinsky
  • Dominika Ďurechová

Keywords:

Populus sp., in vitro culture, microclonal propagation, regeneration, kanamycin and phosphinothricin selection

Abstract

Poplars (genus Populus) have emerged as a model organism for forest biotechnology, and genetic modification is more advanced for this genus than for any other tree. So far several protocols for microclonal propagation and regeneration for Populus species have been developed. However it is well known that these protocols differ for various species and need to be adapted even for different clones of the same species. This work was focused on developing of protocols for propagation, regeneration and putative transformant´s selection of aspen Populus tremula L. and other two fast-growing Populus species (P. nigra L., P. x canadensis Moench). The regeneration ability for black poplar explants was demonstrated to be three times higher compared to those for aspen and hybrid poplar. It was found that concentration 1 mg/L of phosphinothricin and 25 mg/L of kanamycin is toxic for non- transgenic plant tissues of P. x canadensis and can be applied in transformation experiments when genes of resistance to the corresponding selective agents into the plant genome are introduced.

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Published

2013-02-01

How to Cite

Kutsokon, N., Libantova, J., Rudas, V., Rashydov, N., Grodzinsky, D., & Ďurechová, D. (2013). ADVANCING PROTOCOLS FOR POPLARS in vitro PROPAGATION, REGENERATION AND SELECTION OF TRANSFORMANTS . Journal of Microbiology, Biotechnology and Food Sciences, 2(special issue 2), 1447–1454. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7475

Issue

Vol. 2 No. special issue 2 (2013): Special issue 1 - part A

Section

Biotechnology

License

Copyright (c) 2013 Nataliia Kutsokon, Jana Libantova, Volodymyr Rudas, Namik Rashydov, Dmytro Grodzinsky, Dominika Ďurechová

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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