ISOLATION AND CHARACTERIZATION OF CHITINASE GENE FROM THE UNTRADITIONAL PLANT SPECIES
Keywords:
carnivorous plant, chitinase, in silico analyses, sundewAbstract
Round-leaf sundew (Drosera rotundifolia L.) from Droseraceae family belongs among a few plant species with strong antifungal potential. It was previously shown that chitinases of carnivorous plant species may play role during the insect prey digestion, when hard chitin skeleton is being decomposed. As many phytopathogenic fungi contain chitin in their cell wall our attention in this work was focused on isolation and in silico characterization of genomic DNA sequence of sundew chitinase gene. Subsequently this gene was fused to strong constitutive CaMV35S promoter and cloned into the plant binary vector pBinPlus and tested in A. tumefaciens LBA 4404 for its stability. Next, when transgenic tobacco plants are obtained, increasing of their antifungal potential will be tested.Downloads
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Published
2013-02-01
How to Cite
ÄŽurechová, D., MatuÅ¡Ãková, I., MoravÄÃková, J., JopÄÃk, M., & Libantová, J. (2013). ISOLATION AND CHARACTERIZATION OF CHITINASE GENE FROM THE UNTRADITIONAL PLANT SPECIES. Journal of Microbiology, Biotechnology and Food Sciences, 2(special issue 2), 2208–2216. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7461
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Section
Biotechnology
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Copyright (c) 2013 Dominika ÄŽurechová, Ildikó MatuÅ¡Ãková, Jana MoravÄÃková, Martin JopÄÃk, Jana Libantová
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