PREPARATION OF PLANT VECTOR CONSTRUCT CONTAINING DEHYDRIN GENE At2g21490
DOI:
https://doi.org/10.15414/jmbfs.2017.6.6.1261-1263Keywords:
Agrobacterium tumefaciens, Arabidopsis thaliana, At2g21490, binary vector, dehydrinsAbstract
This work is focused on preparation of a plant transformation vector pDH2 containing the expression unit of the dehydrin gene At2g21490 under a control of the constitutive dCaMV 35S promoter and the selectable marker neomycin phosphotransferase gene controlled by the nos promoter. The gene At2g21490 is classified as a dehydrin of the type K2SY2. The gene was isolated from Arabidopsis thaliana by PCR approach. The plasmid pDH2 was transformed into the strain Agrobacterium tumefaciens LBA 4404. The stability of the plasmid pDH2 in Agrobacterium has been verified by restriction analysis after re-transformation of the pDH2 into Escherichia coli.Downloads
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Published
2017-06-01
How to Cite
Zimová, M., Boszorádová, E., Gregorová, Z., MatuÅ¡Ãková, I., & MoravÄÃková, J. (2017). PREPARATION OF PLANT VECTOR CONSTRUCT CONTAINING DEHYDRIN GENE At2g21490. Journal of Microbiology, Biotechnology and Food Sciences, 6(6), 1261–1263. https://doi.org/10.15414/jmbfs.2017.6.6.1261-1263
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Biotechnology
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Copyright (c) 2017 Mária Zimová, Eva Boszorádová, Zuzana Gregorová, Ildikó MatuÅ¡Ãková, Jana MoravÄÃková
This work is licensed under a Creative Commons Attribution 4.0 International License.
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