2-DE PROTEOME MAPS OF AMARANTH AND BUCKWHEAT SEEDS

Abstract

Our work was focus on amaranth (Amaranthus sp.) cv. Plaisman and buckwheat (Fagopyrum Mill.), cv. Pyra proteome, which was analysed by 2-D electrophoresis. We found similarity between the chemical properties proteins of pseudocereals amaranth and buckwheat. Image analysis showed a higher number of spots on 2-DE map of buckwheat in comparison to amaranth. Some similarities were in protein spots at approximately 21,000 Da, pI 7 and strip of protein spots in range of pI 8-10, 21,000 Da. The buckwheat 2-DE map shows spots of protein with higher intensity in the region ranging from 30-45,000 Da, pI 5-6 as well as highly abundant protein spots from visible at 36-40,000 Da, pI 8-9. Protein maps showed that the pseudocereals do not content storage proteins, which indicates that they are suitable as a replacement for cereals for people with celiac disease.