PLANT TRANSFORMATION VECTORS AND THEIR STABILITY IN AGROBACTERIUM TUMEFACIENS

Authors

  • Zuzana Polóniová
  • Pavol Dubnický
  • Zdenka Gálová
  • Jana Libantová
  • Ildikó Matušíková
  • Jana Moravčíková

Keywords:

Agrobacterium tumefaciens, plant transformation vector

Abstract

The stability of the plant transformation vectors pTS2, pEV2 and pEV8 was tested in two Agrobacterium tumefaciens strains LBA 4404 and C58C1 that differed in chromosomal background. The T-DNAs of all binary vectors contained the β-glucuronidase reporter and the neomycin phosphotransferase selectable marker genes. In addition, the plasmids pEV2 and pEV8 contained the Cre/loxP recombination system. The binary vectors were transformed into A. tumefaciens strains and their stability was evaluated by restriction analyses after retransformation of plasmids from A. tumefaciens into E. coli. Our results showed that plasmids pTS2 and pEV2 were stable in both bacterial strains while the pEV8 exhibited instability in A. tunmefaciens LBA 4404.

Downloads

Download data is not yet available.

Downloads

Published

2013-02-01

How to Cite

Polóniová, Z., Dubnický, P., Gálová, Z., Libantová, J., Matušíková, I., & Moravčíková, J. (2013). PLANT TRANSFORMATION VECTORS AND THEIR STABILITY IN AGROBACTERIUM TUMEFACIENS. Journal of Microbiology, Biotechnology and Food Sciences, 2(special issue 2), 1559–1568. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7487

Most read articles by the same author(s)

1 2 3 4 5 6 7 8 9 > >> 

Similar Articles

You may also start an advanced similarity search for this article.