POLYPHENOL-RICH POMEGRANATE EXTRACT AS A POTENTIAL MODULATOR OF STEROIDOGENESIS IN HUMAN OVARIAN CELLS

Authors

  • Simona Baldovská
  • Katarína Michalcová
  • Marek Halenár
  • Angel A. Carbonell-Barrachina
  • Adriana Kolesárová

DOI:

https://doi.org/10.15414/jmbfs.2019.8.6.1343-1346

Keywords:

pomegranate, ovarian cells, steroid hormones, cancer

Abstract

Pomegranate represents a rich source of phytocompounds with high medicinal value. Nowadays, many studies have shown that the pomegranate extract also possesses anti-oxidant, anti-inflammatory and anti-proliferative effects on cancer cells, thus leading to increased popularity as a functional food and nutraceuticals. The aim of the study was to determine the biological effect of dry pomegranate extract (at concentrations 5, 10, 20 and 40 µg/ml; for 24h) on the viability of ovarian cells and the secretion of steroid hormones. Cultures of human ovarian granulosa cells (HGL5) and human ovarian carcinoma cells (OVCAR-3) were used such as a model cell system. The metabolic activity was evaluated by AlamarBlueTM assay, the release of steroid hormones was assayed by the ELISA method. Experimental results indicated a significant (P≤0.001) increase of proliferation in HGL5 cells after the addition of the extract at the concentrations 5, 10 and 20 µg/ml. Moreover, the number of viable OVCAR-3 cells significantly (P≤0.05; P≤0.01; P≤0.001) decreased after the addition of the extract at the concentrations 10, 20 and 40 µg/ml compared to the control. In addition, the secretion of 17ß-estradiol by the HGL5 cells was significantly (P≤0.05; P≤0.001) increased at all used concentrations of the extract. Despite to increasing of the 17ß-estradiol secretion, progesterone levels produced by the HGL5 cells were not significantly (P≥0.05) affected at all used concentrations of the extract. The current study provided experimental evidence that the pomegranate extract might be a promising candidate as a potential modulator of steroidogenesis and as a potential chemoprotective agent.

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Published

2019-06-01

How to Cite

Baldovská, S., Michalcová, K., Halenár, M., A. Carbonell-Barrachina, A., & Kolesárová, A. (2019). POLYPHENOL-RICH POMEGRANATE EXTRACT AS A POTENTIAL MODULATOR OF STEROIDOGENESIS IN HUMAN OVARIAN CELLS. Journal of Microbiology, Biotechnology and Food Sciences, 8(6), 1343–1346. https://doi.org/10.15414/jmbfs.2019.8.6.1343-1346

Issue

Section

Biotechnology