EFFECTS OF PROPOLIS EXTRACTS IN CHICKENS DIET AGAINST SALMONELLA TYPHIMURIUM DETECTED BY REAL-TIME PCR

Abstract

The aim of this study was to follow the effect of propolis extractsin chickens feed against colonization of GIT (gastrointestinal tract) with Salmonella spp. by Step One real-time PCR. Propolis has been used in folk medicine since ancient times due to its many biological properties, such as antimicrobial, anti-inflammatory, antioxidant, immunomodulatory activities, among others. Propolis extracts was applied to chickens feeds in four groups with different concentration of propolis. We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and MicroSEQ® Salmonella spp. Detection Kit for pursuance the realtime PCR(Applied Biosystems). In the investigated samples without incubation we could detect strain of Salmonella spp. in twenty of twenty five samples (swabs), as well as internal positive control (IPC), which was positive in all samples. Our results indicate positive effect of propolis against colonization of GIT (gastrointestinal tract) with Salmonellaspp. in all experimental groups. This Step One real-time PCR assay is extremely useful for any laboratory equipped by real-time PCR. Thus, these results proved real-time PCR to be useful as a rapid diagnostic test for the direct detection of pathogens in food, without the need of enrichment steps.