THE EFFECT OF DIFFERENT CONCENTRATION OF TREHALOSE ON TURKEY SPERMATOZOA MOTILITY IN VITRO

Abstract

Target of this study was to analyze the effect of a different trehalose concentration (T10 – 10 mg.ml-1; T5 – 5 mg.ml-1; T2.5 – 2.5 mg.ml-1; T1.25 – 1.25 mg.ml-1) on the turkey spermatozoa motility parameters after a culture in vitro at 5°C. For control the semen samples were diluted with physiological solution. Individual parameters were recorded at six time periods: 0, 30, 60, 90, 120, 150, 180 minutes. The highest values of spermatozoa motility were determined in samples containing 10 mg.ml-1 of trehalose. The highest motility of 88.16% was recorded after 30 minutes of culture in the sample T10. Significantly (p<0.01) higher data were detected in T10 samples compared to control after 90 minutes of culture. After 180 minutes of culture a significantly (p<0.01) higher motility was detected in control. In the samples T5; T2.5 and T1.25 similar significantly lower values were recorded compared to control, except for T1.25 at the time of 90 minutes of culture. The highest progressive motility 77.97% was observed in samples T10 after 30 minutes of culture. A significantly lower value in group T10 (p<0.05) was detected at time 180. When compared to other control lower values at of all time periods were found.